ARAF Polyclonal antibody
ARAF Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
WB, IHC, IF, ELISA
Cat no : 22129-1-AP
|Positive WB detected in||HeLa cells, A431 cells, NIH/3T3 cells|
|Positive IHC detected in||human skin cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
|WB||See 2 publications below|
|IHC||See 1 publications below|
|IF||See 1 publications below|
The immunogen of 22129-1-AP is ARAF Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ARAF fusion protein Ag17588|
|Full Name||v-raf murine sarcoma 3611 viral oncogene homolog|
|Calculated molecular weight||609 aa, 68 kDa|
|Observed molecular weight||33 kDa|
|GenBank accession number||BC007514|
|Gene ID (NCBI)||369|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ARAF(Serine/threonine-protein kinase A-Raf) is also named as ARAF1, PKS, PKS2 and belongs to the protein kinase superfamily. It is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It has 2 isoforms produced by alternative splicing and the isoform 2 serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.(PMID:17535970).
|Product Specific Protocols|
|WB protocol for ARAF antibody 22129-1-AP||Download protocol|
|IHC protocol for ARAF antibody 22129-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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