|Positive WB detected in||HeLa cells, A431 cells, NIH/3T3 cells|
|Positive IHC detected in||human skin cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22129-1-AP is ARAF Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ARAF fusion protein Ag17588|
|Full Name||v-raf murine sarcoma 3611 viral oncogene homolog|
|Calculated molecular weight||609 aa, 68 kDa|
|Observed molecular weight||33 kDa|
|GenBank accession number||BC007514|
|Gene ID (NCBI)||369|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ARAF(Serine/threonine-protein kinase A-Raf) is also named as ARAF1, PKS, PKS2 and belongs to the protein kinase superfamily. It is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It has 2 isoforms produced by alternative splicing and the isoform 2 serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.(PMID:17535970).
Novel recombinant protein FlaA N/C increases tumor radiosensitivity via NF-κB signaling in murine breast cancer cells.
Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.
Targeting the p300/NONO axis sensitizes melanoma cells to BRAF inhibitors.