ARAF Polyclonal antibody

ARAF Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 22129-1-AP


A RAF, ARAF, ARAF1, PKS, PKS2, Proto oncogene A Raf, Proto oncogene A Raf 1, Proto oncogene Pks, RAFA1

Tested Applications

Positive WB detected inHeLa cells, A431 cells, NIH/3T3 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22129-1-AP targets ARAF in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARAF fusion protein Ag17588
Full Name v-raf murine sarcoma 3611 viral oncogene homolog
Calculated Molecular Weight 609 aa, 68 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC007514
Gene Symbol ARAF
Gene ID (NCBI) 369
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARAF(Serine/threonine-protein kinase A-Raf) is also named as ARAF1, PKS, PKS2 and belongs to the protein kinase superfamily. It is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It has 2 isoforms produced by alternative splicing and the isoform 2 serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.(PMID:17535970).


Product Specific Protocols
WB protocol for ARAF antibody 22129-1-APDownload protocol
IHC protocol for ARAF antibody 22129-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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