ARAF Polyclonal antibody

ARAF Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 22129-1-AP

Synonyms

A RAF, ARAF, ARAF1, PKS, PKS2, Proto oncogene A Raf, Proto oncogene A Raf 1, Proto oncogene Pks, RAFA1

---

Validation Data Gallery

• 

  WB analysis of HeLa using 22129-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22129-1-AP (ARAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 22129-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22129-1-AP (ARAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 22129-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22129-1-AP (ARAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 22129-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22129-1-AP (ARAF Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 22129-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22129-1-AP (ARAF Antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, NIH/3T3 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 22129-1-AP is ARAF Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARAF fusion protein Ag17588
• Full Name: v-raf murine sarcoma 3611 viral oncogene homolog
• Calculated molecular weight: 609 aa, 68 kDa
• Observed molecular weight: 33 kDa
• GenBank accession number: BC007514
• Gene symbol: ARAF
• Gene ID (NCBI): 369
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARAF(Serine/threonine-protein kinase A-Raf) is also named as ARAF1, PKS, PKS2 and belongs to the protein kinase superfamily. It is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It has 2 isoforms produced by alternative splicing and the isoform 2 serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.(PMID:17535970).

Publications

Species	Application	Title
human	IHC	Oncogene; Targeting the p300/NONO axis sensitizes melanoma cells to BRAF inhibitors.; Authors - Feifei Zhang
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
mouse	WB,IF	Oncol Lett; Novel recombinant protein FlaA N/C increases tumor radiosensitivity via NF-κB signaling in murine breast cancer cells.; Authors - Ying Xu