ARAF Polyclonal antibody

ARAF Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22129-1-AP

Synonyms

A RAF, ARAF, ARAF1, PKS, PKS2, Proto oncogene A Raf, Proto oncogene A Raf 1, Proto oncogene Pks, RAFA1



Tested Applications

Positive WB detected inHeLa cells, A431 cells, NIH/3T3 cells
Positive IHC detected inhuman skin cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22129-1-AP is ARAF Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARAF fusion protein Ag17588
Full Name v-raf murine sarcoma 3611 viral oncogene homolog
Calculated molecular weight 609 aa, 68 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC007514
Gene symbol ARAF
Gene ID (NCBI) 369
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARAF(Serine/threonine-protein kinase A-Raf) is also named as ARAF1, PKS, PKS2 and belongs to the protein kinase superfamily. It is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It has 2 isoforms produced by alternative splicing and the isoform 2 serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.(PMID:17535970).

Protocols

Product Specific Protocols
WB protocol for ARAF antibody 22129-1-APDownload protocol
IHC protocol for ARAF antibody 22129-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Oncol Lett

Novel recombinant protein FlaA N/C increases tumor radiosensitivity via NF-κB signaling in murine breast cancer cells.

Authors - Ying Xu