Arfaptin-1 Monoclonal antibody

Arfaptin-1 Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

CloneNo.

2A10B3

Cat no : 67461-1-Ig

Synonyms

Arfaptin 1, Arfaptin-1, ARFIP1, HSU52521



Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67461-1-Ig targets Arfaptin-1 in WB, IHC, FC,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Arfaptin-1 fusion protein Ag12097
Full Name ADP-ribosylation factor interacting protein 1
Calculated molecular weight 373 aa, 42 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC103759
Gene symbol ARFIP1
Gene ID (NCBI) 27236
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Arfaptin-1 antibody 67461-1-IgDownload protocol
IHC protocol for Arfaptin-1 antibody 67461-1-IgDownload protocol
FC protocol for Arfaptin-1 antibody 67461-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols