Arfaptin-1 Monoclonal antibody

Arfaptin-1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2A10B3

Cat no : 67461-1-Ig

Synonyms

Arfaptin 1, Arfaptin-1, ARFIP1, HSU52521

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Validation Data Gallery

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  WB analysis using 67461-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67461-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67461-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 67461-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 67461-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67461-1-Ig (Arfaptin-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 67461-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67461-1-Ig (Arfaptin-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 67461-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse kidney using 67461-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 67461-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of A549 using 67461-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of Jurkat using 67461-1-Ig

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human Arfaptin-1 (67461-1-Ig, Clone:2A10B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (black). Cells were fixed with 90% MeOH .

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Tested Applications

• Positive WB detected in: HeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, HEK-293 cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse kidney tissue, HeLa cells, A549 cells
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Product Information

67461-1-Ig targets Arfaptin-1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Arfaptin-1 fusion protein Ag12097
• Full Name: ADP-ribosylation factor interacting protein 1
• Calculated molecular weight: 373 aa, 42 kDa
• Observed molecular weight: 38-42 kDa
• GenBank accession number: BC103759
• Gene symbol: ARFIP1
• Gene ID (NCBI): 27236
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.