Arfaptin-1 Monoclonal antibody
Arfaptin-1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
2A10B3
Cat no : 67461-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, RAW 264.7 cells, HepG2 cells, Jurkat cells |
Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse kidney tissue, HeLa cells, A549 cells |
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Product Information
67461-1-Ig targets Arfaptin-1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Arfaptin-1 fusion protein Ag12097 |
Full Name | ADP-ribosylation factor interacting protein 1 |
Calculated molecular weight | 373 aa, 42 kDa |
Observed molecular weight | 38-42 kDa |
GenBank accession number | BC103759 |
Gene symbol | ARFIP1 |
Gene ID (NCBI) | 27236 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for Arfaptin-1 antibody 67461-1-Ig | Download protocol |
IHC protocol for Arfaptin-1 antibody 67461-1-Ig | Download protocol |
IF protocol for Arfaptin-1 antibody 67461-1-Ig | Download protocol |
FC protocol for Arfaptin-1 antibody 67461-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |