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- KD/KO Validated
Arginase-1 Monoclonal antibody
Arginase-1 Monoclonal Antibody for IHC, IP, WB,ELISA
Cat no : 66129-1-Ig
|Positive WB detected in||rat liver tissue, RAW 264.7 cells, mouse liver tissue, pig liver tissue|
|Positive IP detected in||rat liver tissue|
|Positive IHC detected in||human liver cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:1000|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66129-1-Ig is Arginase-1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, pig, rat, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Arginase-1 fusion protein Ag8810|
|Full Name||arginase, liver|
|Calculated molecular weight||236aa,25 kDa; 322aa,35 kDa|
|Observed molecular weight||36 kDa|
|GenBank accession number||BC005321|
|Gene ID (NCBI)||383|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins (PMID: 20071539, PMID:12098359). It can exsit as a homotrimer and it has 3 isoforms produced by alternative splicing (PMID:16141327). Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control (PMID:27117406). ARG1 mainly expresses in neurons in a normal brain. The expression of ARG1 increases in microglia/macrophages and astrocytes early after CNS injuries. ARG1 has been regarded as a marker for beneficial microglia/macrophages and possesses antiinflammatory and tissue repair properties under various pathological conditions (PMID: 26538310, PMID: 31619589).
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