CoraLite®488-conjugated Arginase-1 Monoclonal antibody

Arginase-1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat, mouse

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

5D6D12

Cat no : CL488-66129

Synonyms

ARG1, Arginase 1, arginase, liver, Arginase-1, Liver type arginase, Type I arginase



Tested Applications

Positive IF detected inHepG2 cells, HeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66129-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-66129 is Arginase-1 Fusion Protein expressed in E. coli.

Tested Reactivity human, pig, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Arginase-1 fusion protein Ag8810
Full Name arginase, liver
Calculated molecular weight236aa,25 kDa; 322aa,35 kDa
GenBank accession numberBC005321
Gene symbol ARG1
Gene ID (NCBI) 383
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation(PMID:12098359) and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins(PMID: 20071539).It can exsit as a homotrimer(PMID:16141327) and it has 3 isoforms produced by alternative splicing.Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control.(PMID:27117406). ARG1 is a novel biomarker of post-stroke immune suppression(2016). The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
IF protocol for CL488 Arginase-1 antibody CL488-66129Download protocol
Standard Protocols
Click here to view our Standard Protocols