CoraLite®488-conjugated Arginase-1 Monoclonal antibody

Arginase-1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat, mouse

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

5D6D12

Cat no : CL488-66129

Synonyms

ARG1, Arginase 1, arginase, liver, Arginase-1, Liver type arginase, Type I arginase



Tested Applications

Positive IF detected inHepG2 cells, HeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66129-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

The immunogen of CL488-66129 is Arginase-1 Fusion Protein expressed in E. coli.

Tested Reactivity human, pig, rat, mouse
Cited Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Arginase-1 fusion protein Ag8810
Full Name arginase, liver
Calculated molecular weight236aa,25 kDa; 322aa,35 kDa
GenBank accession numberBC005321
Gene symbol ARG1
Gene ID (NCBI) 383
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation(PMID:12098359) and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins(PMID: 20071539).It can exsit as a homotrimer(PMID:16141327) and it has 3 isoforms produced by alternative splicing.Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control.(PMID:27117406). ARG1 is a novel biomarker of post-stroke immune suppression(2016). The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
IF protocol for CL488 Arginase-1 antibody CL488-66129Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Biosci Rep

Reduction of TMAO level enhances the stability of carotid atherosclerotic plaque through promoting macrophage M2 polarization and efferocytosis.

Authors - Weihao Shi