Arginase-1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Arginase-1 Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat, mouse

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5D6D12

Cat no : 66129-1-PBS

Synonyms

ARG1, Arginase 1, arginase, liver, Arginase-1, liver Arginase, Liver type arginase

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Validation Data Gallery

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  WB analysis of rat liver using 66129-1-Ig (same clone as 66129-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66129-1-Ig (ARG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66129-1-Ig (ARG1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 66129-1-Ig (same clone as 66129-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66129-1-Ig (ARG1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 66129-1-Ig (same clone as 66129-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66129-1-Ig (ARG1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 66129-1-Ig (same clone as 66129-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66129-1-Ig (ARG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

• 

  IP experiment of rat liver using 66129-1-Ig (same clone as 66129-1-PBS)

  IP result of anti-Arginase-1 (IP:66129-1-Ig, 5ug; Detection:66129-1-Ig 1:1000) with rat liver tissue lysate 5520 ug. This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66129-1-Ig (Arginase-1 antibody) at dilution of 1:4800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66129-1-Ig (Arginase-1 antibody) at dilution of 1:4800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Arginase-1 antibody (66129-1-Ig, Clone: 5D6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 66129-1-Ig (same clone as 66129-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Arginase-1 antibody (66129-1-Ig, Clone: 5D6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66129-1-Ig (same clone as 66129-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Arginase-1 antibody (66129-1-Ig, Clone: 5D6D12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66129-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66129-1-PBS targets Arginase-1 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human, pig, rat, mouse samples.

• Tested Reactivity: human, pig, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Arginase-1 fusion protein Ag8810
• Full Name: arginase, liver
• Calculated Molecular Weight: 236aa,25 kDa; 322aa,35 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC005321
• Gene Symbol: ARG1
• Gene ID (NCBI): 383
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins (PMID: 20071539, PMID:12098359). It can exsit as a homotrimer and it has 3 isoforms produced by alternative splicing (PMID:16141327). Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control (PMID:27117406). Before stroke, ARG1 mainly expressed in neurons in a normal brain (PMID: 23311438). The expression of ARG1 increases in microglia/macrophages and astrocytes early after CNS injuries. ARG1 has been regarded as a marker for beneficial microglia/macrophages and possesses antiinflammatory and tissue repair properties under various pathological conditions (PMID: 26538310, PMID: 31619589).