ARHGDIG Polyclonal antibody

ARHGDIG Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14282-1-AP

Synonyms

ARHGDIG, Rho GDI 3, Rho GDI gamma, RHOGDI 3

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Validation Data Gallery

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  WB analysis using 14282-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14282-1-AP (ARHGDIG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14282-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14282-1-AP (ARHGDIG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 14282-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14282-1-AP (ARHGDIG antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 14282-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14282-1-AP (ARHGDIG antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human brain tissue, HeLa cells, Jurkat cells, MCF-7 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14282-1-AP targets ARHGDIG in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARHGDIG fusion protein Ag5554
• Full Name: Rho GDP dissociation inhibitor (GDI) gamma
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC047699
• Gene Symbol: ARHGDIG
• Gene ID (NCBI): 398
• RRID: AB_2059733
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Oncol Rep; Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer.; Authors - Hao Bo