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  • KD/KO Validated

ARHGEF4 Antibody

ARHGEF4 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 55213-1-AP

Synonyms

ARHGEF4, ASEF, ASEF1, GEF4, KIAA1112, STM6



Tested Applications

Positive WB detected inmouse brain tissue, multi-cells/tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

55213-1-AP targets ARHGEF4 in IHC, IP, WB, ELISA applications and shows reactivity with human, mouse samples.

Reactivity human, mouse
Cited Species human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name Rho guanine nucleotide exchange factor (GEF) 4
Calculated molecular weight 79 kDa
Observed molecular weight 72 kDa
GenBank accession number NM_015320
Gene symbol ARHGEF4
Gene ID (NCBI) 50649
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

ARHGEF4, also named as KIAA1112 and Asef, acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC, ARHGEF4 may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion.(PMID: 10873612). This antibody is specific to ARHGEF4.

Protocols

Product Specific Protocols
WB protocol for ARHGEF4 antibody 55213-1-APDownload protocol
IHC protocol for ARHGEF4 antibody 55213-1-APDownload protocol
IP protocol for ARHGEF4 antibody 55213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Oncotarget

Efficient delivery of small interfering RNAs targeting particular mRNAs into pancreatic cancer cells inhibits invasiveness and metastasis of pancreatic tumors.

Authors - Keisuke Taniuchi

Int J Oncol

ARHGEF4 predicts poor prognosis and promotes cell invasion by influencing ERK1/2 and GSK-3α/β signaling in pancreatic cancer.

Authors - Keisuke Taniuchi
human,mouseIHC,IF

J Neurosci

Temporal Profiling of Astrocyte Precursors Reveals Parallel Roles for Asef during Development and after Injury.

Authors - Lesley S Chaboub
  • KO Validated
humanCoIP

Nat Chem Biol

Peptidomimetic inhibitors of APC-Asef interaction block colorectal cancer migration.

Authors - Haiming Jiang