ARHGEF4 Polyclonal antibody

• Featured Product
• KD/KO Validated

ARHGEF4 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 55213-1-AP

Synonyms

ARHGEF4, ASEF, ASEF1, GEF4, KIAA1112, STM6

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Validation Data Gallery

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  WB analysis of mouse brain using 55213-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55213-1-AP (ARHGEF4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of multi-cells/tissue using 55213-1-AP

  WB result of 55213-1-AP (ARHGEF4 Antibody).

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  IP experiment of mouse brain using 55213-1-AP

  IP result of anti-ARHGEF4 (IP:55213-1-AP, 4ug; Detection:55213-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human brain using 55213-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55213-1-AP (ARHGEF4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 55213-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55213-1-AP (ARHGEF4 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, multi-cells/tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Product Information

55213-1-AP targets ARHGEF4 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Rho guanine nucleotide exchange factor (GEF) 4
• Calculated Molecular Weight: 79 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: NM_015320
• Gene Symbol: ARHGEF4
• Gene ID (NCBI): 50649
• RRID: AB_2721023
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGEF4, also named as KIAA1112 and Asef, acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC, ARHGEF4 may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion.(PMID: 10873612). This antibody is specific to ARHGEF4.

Publications

Species	Application	Title
human	CoIP	Nat Chem Biol; Peptidomimetic inhibitors of APC-Asef interaction block colorectal cancer migration.; Authors - Haiming Jiang
human,mouse	IHC,IF	J Neurosci; Temporal Profiling of Astrocyte Precursors Reveals Parallel Roles for Asef during Development and after Injury.; Authors - Lesley S Chaboub ; KO Validated
human	IHC	iScience; Whole-genome DNA methylation and DNA methylation-based biomarkers in lung squamous cell carcinoma; Authors - Qidong Cai
		Int J Oncol; ARHGEF4 predicts poor prognosis and promotes cell invasion by influencing ERK1/2 and GSK-3α/β signaling in pancreatic cancer.; Authors - Keisuke Taniuchi
mouse	WB	Biol Reprod; Raptor directs Sertoli cell cytoskeletal organization and polarity in the mouse testis.; Authors - Zhi Xiong
human	WB	Cell Adh Migr; ARHGEF4-mediates the actin cytoskeleton reorganization of hepatic stellate cells in 3-dimensional collagen matrices.; Authors - Xiaowei Zhang ; KD Validated