|Positive WB detected in||mouse brain tissue, multi-cells/tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
55213-1-AP targets ARHGEF4 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||Rho guanine nucleotide exchange factor (GEF) 4|
|Calculated molecular weight||79 kDa|
|Observed molecular weight||72 kDa|
|GenBank accession number||NM_015320|
|Gene ID (NCBI)||50649|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
ARHGEF4, also named as KIAA1112 and Asef, acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC, ARHGEF4 may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion.(PMID: 10873612). This antibody is specific to ARHGEF4.
Efficient delivery of small interfering RNAs targeting particular mRNAs into pancreatic cancer cells inhibits invasiveness and metastasis of pancreatic tumors.
Int J Oncol
ARHGEF4 predicts poor prognosis and promotes cell invasion by influencing ERK1/2 and GSK-3α/β signaling in pancreatic cancer.
Temporal Profiling of Astrocyte Precursors Reveals Parallel Roles for Asef during Development and after Injury.
Nat Chem Biol
Peptidomimetic inhibitors of APC-Asef interaction block colorectal cancer migration.