Validation Data Gallery
|Positive WB detected in||MCF-7 cells, A549 cells, K-562 cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human testis tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 24499-1-AP is ARID4B Fusion Protein expressed in E. coli.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ARID4B fusion protein Ag21462|
|Full Name||AT rich interactive domain 4B (RBP1-like)|
|Calculated molecular weight||1312 aa, 148 kDa|
|Observed molecular weight||100 kDa, 200 kDa|
|GenBank accession number||BC130418|
|Gene ID (NCBI)||51742|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ARID4B, also named as BRCAA1 or SAP180, is a 1312 amino acid protein, which contains one ARID domain. ARID4B localizes in the nucleus and cytoplasm. ARID4B as a transcription repressor may function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. ARID4B is highly expressed in the testis and in breast, lung, colon, pancreatic and ovarian cancers. Expressed at low levels in the thymus, prostate and ovary.
|Product Specific Protocols|
|WB protocol for ARID4B antibody 24499-1-AP||Download protocol|
|IHC protocol for ARID4B antibody 24499-1-AP||Download protocol|
|IF protocol for ARID4B antibody 24499-1-AP||Download protocol|
|IP protocol for ARID4B antibody 24499-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Br J Nutr
Taurine stimulates protein synthesis and proliferation of C2C12 myoblast cells through the PI3K-ARID4B-mTOR pathway.
Cell Death Dis
DNA-methylation-mediated silencing of miR-486-5p promotes colorectal cancer proliferation and migration through activation of PLAGL2/IGF2/β-catenin signal pathways.
SLC14A1 prevents oncometabolite accumulation and recruits HDAC1 to transrepress oncometabolite genes in urothelial carcinoma.
J Clin Invest
The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.