ARID4B Polyclonal antibody

ARID4B Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Cat no : 24499-1-AP

Synonyms

ARID4B, BRCAA1, RBBP1L1, RBP1L1, SAP180



Tested Applications

Positive WB detected inMCF-7 cells, A549 cells, K-562 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24499-1-AP is ARID4B Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARID4B fusion protein Ag21462
Full Name AT rich interactive domain 4B (RBP1-like)
Calculated molecular weight 1312 aa, 148 kDa
Observed molecular weight 100 kDa, 200 kDa
GenBank accession numberBC130418
Gene symbol ARID4B
Gene ID (NCBI) 51742
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARID4B, also named as BRCAA1 or SAP180, is a 1312 amino acid protein, which contains one ARID domain. ARID4B localizes in the nucleus and cytoplasm. ARID4B as a transcription repressor may function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. ARID4B is highly expressed in the testis and in breast, lung, colon, pancreatic and ovarian cancers. Expressed at low levels in the thymus, prostate and ovary.

Protocols

Product Specific Protocols
WB protocol for ARID4B antibody 24499-1-APDownload protocol
IHC protocol for ARID4B antibody 24499-1-APDownload protocol
IF protocol for ARID4B antibody 24499-1-APDownload protocol
IP protocol for ARID4B antibody 24499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Br J Nutr

Taurine stimulates protein synthesis and proliferation of C2C12 myoblast cells through the PI3K-ARID4B-mTOR pathway.

Authors - Qi Hao
  • KD Validated
humanWB

Cell Death Dis

DNA-methylation-mediated silencing of miR-486-5p promotes colorectal cancer proliferation and migration through activation of PLAGL2/IGF2/β-catenin signal pathways.

Authors - Xiangxiang Liu
humanWB

Theranostics

SLC14A1 prevents oncometabolite accumulation and recruits HDAC1 to transrepress oncometabolite genes in urothelial carcinoma.

Authors - Ti-Chun Chan
humanWB

J Clin Invest

The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.

Authors - Wanjin Li