ASB14 Polyclonal antibody

ASB14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25300-1-AP

Synonyms

ASB 14, ASB14

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Validation Data Gallery

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  WB analysis of mouse spleen using 25300-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25300-1-AP (ASB14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 25300-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25300-1-AP (ASB14 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 25300-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25300-1-AP (ASB14 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 25300-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25300-1-AP (ASB14 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse spleen tissue, Raji cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25300-1-AP is ASB14 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASB14 fusion protein Ag18147
• Full Name: ankyrin repeat and SOCS box-containing 14
• Calculated Molecular Weight: 587 aa, 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC114953
• Gene Symbol: ASB14
• Gene ID (NCBI): 142686
• RRID: AB_2880017
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.