ASF1A Polyclonal antibody

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• ASF1A Polyclonal antibody (10784-1-AP)

ASF1A Polyclonal Antibody for WB, ELISA

Cat No. 10784-1-AP Copied!

Publications (6)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Anti-silencing function protein 1 homolog A, CCG1 interacting factor A, CCG1-interacting factor A, CGI 98, CGI-98

• 

  WB analysis of HeLa using 10784-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10784-1-AP (ASF1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 10784-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10784-1-AP (ASF1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10784-1-AP targets ASF1A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1233

  Product name: Recombinant human ASF1A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-204 aa of BC010878

  Sequence: MAKVQVNNVVVLDNPSPFYNPFQFEITFECIEDLSEDLEWKIIYVGSAESEEYDQVLDSVLVGPVPAGRHMFVFQADAPNPGLIPDADAVGVTVVLITCTYRGQEFIRVGYYVNNEYTETELRENPPVKPDFSKLQRNILASNPRVTRFHINWEDNTEKLEDAESSNPNLQSLLSTDALPSASKGWSTSENSLNVMLESHMDCM

• Full Name: ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 204 aa, 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC010878
• Gene Symbol: ASF1A
• Gene ID (NCBI): 25842
• RRID: AB_2243015
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y294
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.

Publications

What published studies show

The anti-ASF1A antibody (Cat# 10784-1-AP) has 6 total citations published in Frontiers in Pharmacology, Biology of Procedures Online, Scientific Reports, Epigenetics & Chromatin, Journal of Virology, and PLoS ONE. Research fields span hepatocellular carcinoma (prognostic signatures and drug sensitivity), histone chaperone biology, pre-implantation embryonic development, virology (varicella-zoster virus), and telomere biology (ALT-associated PML bodies). Applications include WB, IHC, IF, and IP in human and mouse samples.

Automatically generated from the 6 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Front Pharmacol; Prognostic characteristics and drug sensitivity analysis of hepatocellular carcinoma based on histone modification-related genes: a multi-omics integrated study revealing potential therapeutic targets and individualized treatment strategies; Authors - Ping Sun
human	WB,IHC	Sci Rep; Identification of ASF1A and HJURP by global H3-H4 histone chaperone analysis as a prognostic two-gene model in hepatocellular carcinoma; Authors - Yongkang Liu
human	WB	Biol Proced Online; A novel telomere-associated genes signature for the prediction of prognosis and treatment responsiveness of hepatocellular carcinoma; Authors - Kuo Kang
mouse	WB,IF	Epigenetics Chromatin; Distinct role of histone chaperone Asf1a and Asf1b during fertilization and pre-implantation embryonic development in mice.; Authors - Xuemei Wang
human	WB,IP,IF	J Virol; Varicella-zoster virus immediate-early 63 protein interacts with human antisilencing function 1 protein and alters its ability to bind histones h3.1 and h3.3.; Authors - Ambagala Aruna P AP
human	IF	PLoS One; HP1-mediated formation of alternative lengthening of telomeres-associated PML bodies requires HIRA but not ASF1a.; Authors - Jiang Wei-Qin WQ