|Positive WB detected in||HeLa cells, HEK-293 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10784-1-AP targets ASF1A in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||ASF1A fusion protein Ag1233|
|Full Name||ASF1 anti-silencing function 1 homolog A (S. cerevisiae)|
|Calculated molecular weight||204 aa, 23 kDa|
|Observed molecular weight||23 kDa|
|GenBank accession number||BC010878|
|Gene ID (NCBI)||25842|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.
HP1-mediated formation of alternative lengthening of telomeres-associated PML bodies requires HIRA but not ASF1a.
Varicella-zoster virus immediate-early 63 protein interacts with human antisilencing function 1 protein and alters its ability to bind histones h3.1 and h3.3.