ASF1A Polyclonal antibody

ASF1A Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10784-1-AP

Synonyms

ASF1A, CCG1 interacting factor A, CGI 98, CIA, hAsf1, hAsf1a, hCIA, Histone chaperone ASF1A, HSPC146



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10784-1-AP targets ASF1A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASF1A fusion protein Ag1233
Full Name ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
Calculated molecular weight 204 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC010878
Gene symbol ASF1A
Gene ID (NCBI) 25842
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.

Protocols

Product Specific Protocols
WB protocol for ASF1A antibody 10784-1-APDownload protocol
IHC protocol for ASF1A antibody 10784-1-APDownload protocol
IP protocol for ASF1A antibody 10784-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

PLoS One

HP1-mediated formation of alternative lengthening of telomeres-associated PML bodies requires HIRA but not ASF1a.

Authors - Jiang Wei-Qin WQ
humanWB,IF,IP

J Virol

Varicella-zoster virus immediate-early 63 protein interacts with human antisilencing function 1 protein and alters its ability to bind histones h3.1 and h3.3.

Authors - Ambagala Aruna P AP