ASF1A Polyclonal antibody

ASF1A Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10784-1-AP

Synonyms

ASF1A, CCG1 interacting factor A, CGI 98, CIA, hAsf1, hAsf1a, hCIA, Histone chaperone ASF1A, HSPC146

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Validation Data Gallery

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  WB analysis of HeLa using 10784-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10784-1-AP (ASF1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10784-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10784-1-AP (ASF1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10784-1-AP

  IP result of anti-ASF1A (IP:10784-1-AP, 4ug; Detection:10784-1-AP 1:800) with HEK-293 cells lysate 2800ug.

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  IHC staining of human lymphoma using 10784-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10784-1-AP (ASF1A antibody) at dilution of 1:10 (under 10x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

10784-1-AP targets ASF1A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASF1A fusion protein Ag1233
• Full Name: ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 204 aa, 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC010878
• Gene Symbol: ASF1A
• Gene ID (NCBI): 25842
• RRID: AB_2243015
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.

Publications

Species	Application	Title
human	WB,IF,IP	J Virol; Varicella-zoster virus immediate-early 63 protein interacts with human antisilencing function 1 protein and alters its ability to bind histones h3.1 and h3.3.; Authors - Ambagala Aruna P AP
mouse	WB,IF	Epigenetics Chromatin; Distinct role of histone chaperone Asf1a and Asf1b during fertilization and pre-implantation embryonic development in mice.; Authors - Xuemei Wang
human	IF	PLoS One; HP1-mediated formation of alternative lengthening of telomeres-associated PML bodies requires HIRA but not ASF1a.; Authors - Jiang Wei-Qin WQ