CoraLite® Plus 488-conjugated ASF1A Monoclonal antibody

ASF1A Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG3

Reactivity

Human, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D4A6

Cat no : CL488-66884

Synonyms

ASF1A, CCG1 interacting factor A, CGI 98, CIA, DKFZp547E2110, hAsf1, hAsf1a, hCIA, Histone chaperone ASF1A, HSPC146



Tested Applications

Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66884 targets ASF1A in IF applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen ASF1A fusion protein Ag1233
Full Name ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
Calculated Molecular Weight 204 aa, 23 kDa
Observed Molecular Weight 21-23 kDa
GenBank Accession NumberBC010878
Gene Symbol ASF1A
Gene ID (NCBI) 25842
RRIDAB_2883389
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ASF1A antibody CL488-66884Download protocol
Standard Protocols
Click here to view our Standard Protocols