CoraLite® Plus 488-conjugated ASF1A Monoclonal antibody
ASF1A Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG3
Reactivity
Human, rat
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2D4A6
Cat no : CL488-66884
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-66884 targets ASF1A in IF applications and shows reactivity with Human, rat samples.
Tested Reactivity | Human, rat |
Host / Isotype | Mouse / IgG3 |
Class | Monoclonal |
Type | Antibody |
Immunogen | ASF1A fusion protein Ag1233 |
Full Name | ASF1 anti-silencing function 1 homolog A (S. cerevisiae) |
Calculated molecular weight | 204 aa, 23 kDa |
Observed molecular weight | 21-23 kDa |
GenBank accession number | BC010878 |
Gene symbol | ASF1A |
Gene ID (NCBI) | 25842 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 ASF1A antibody CL488-66884 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |