ASIC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ASIC1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 27235-1-AP

Synonyms

ACCN2, Acid sensing ion channel 1, ASIC, ASIC1, ASIC1A, BNaC2, Brain sodium channel 2, hBNaC2

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Validation Data Gallery

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  WB analysis using 27235-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27235-1-AP (ASIC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 27235-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 27235-1-AP (ASIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 27235-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27235-1-AP (ASIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 27235-1-AP

  IP result of anti-ASIC1 (IP:27235-1-AP, 4ug; Detection:27235-1-AP 1:300) with mouse brain tissue lysate 3000ug.

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  IHC staining of human pancreas cancer using 27235-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27235-1-AP (ASIC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 27235-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27235-1-AP (ASIC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 27235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27235-1-AP (ASIC1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27235-1-AP (ASIC1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human pancreas cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 27235-1-AP is ASIC1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASIC1 fusion protein Ag25941
• Full Name: amiloride-sensitive cation channel 2, neuronal
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60-70 kDa, 80-90 kDa
• GenBank Accession Number: NM_001095
• Gene Symbol: ASIC1
• Gene ID (NCBI): 41
• RRID: AB_2880814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASIC1(Acid-sensing ion channel 1), also named as ACCN2, BNaC2 or amiloride-sensitive cation channel 2, is a member of the ASICs family. ASICs, an H+-gated subgroup of the epithelial Na+ channel/degenerin (ENaC/DEG) superfamily, are widely expressed throughout the central and peripheral nervous system and triggered by increased extracellular acidification (PMID: 28518134, 27941930). ASIC1 is a key player in acidosis-induced tumor growth and invasiveness. Clinically, alterations of ASIC1 are associated with poor survival in breast cancer (PMID: 26686084).

Publications

Species	Application	Title
mouse	WB,IF	Research (Wash D C); Targeting ASIC1a Promotes Neural Progenitor Cell Migration and Neurogenesis in Ischemic Stroke; Authors - Hongfei Ge
rat	WB	Cell Signal; Paeoniflorin inhibits pyroptosis of nucleus pulposus cells in an acidic environment and alleviates the degeneration of the intervertebral disc in rats; Authors - Wang-Ying Dai
human	IHC,FC	Lab Invest; Acidosis induces synovial fibroblasts to release vascular endothelial growth factor via acid-sensitive ion channel 1a.; Authors - Xuewen Qian
mouse	FC	Lab Invest; ASIC1a promotes the proliferation of synovial fibroblasts via the ERK/MAPK pathway.; Authors - Jingjing Tao
mouse	WB	Eur J Pharmacol; The interaction of ASIC1a and ERS mediates nerve cell apoptosis induced by insulin deficiency.; Authors - Xuesheng Pan ; KD Validated
mouse	WB	Endocrinology; Knockdown of Acid-sensing Ion Channel 1a in the PVN Promotes Metabolic Disturbances in Male Mice; Authors - Wei Wang