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ASPH Polyclonal antibody

ASPH Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 14116-1-AP


AAH, ASP beta hydroxylase, aspartate beta hydroxylase, ASPH, BAH, CASQ2BP1, HAAH, JCTN, junctin

Tested Applications

Positive WB detected inmouse brain tissue, A549 cells, human brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14116-1-AP targets ASPH in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASPH fusion protein Ag5265
Full Name aspartate beta-hydroxylase
Calculated molecular weight 86 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC066929
Gene symbol ASPH
Gene ID (NCBI) 444
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aspartate-β-hydroxylase (ASPH; termed AAH in older literature) has been implicated in the cross-talk among all of these signaling pathways. Correspondingly, ASPH is expressed at high levels in many malignant neoplasms of different histogeneses. It has the 86-141 kDa bands (native and phosphorylated forms), cleavage products of 35-56 and 22-26 kDa.(PMID:27981247/PMID: 10956665)


Product Specific Protocols
WB protocol for ASPH antibody 14116-1-APDownload protocol
IHC protocol for ASPH antibody 14116-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Immunol Res

Construction and Characterization of Adenovirus Vectors Encoding Aspartate-β-Hydroxylase to Preliminary Application in Immunotherapy of Hepatocellular Carcinoma.

Authors - Yujiao Zhou


Hydroxylase Activity of ASPH Promotes Hepatocellular Carcinoma Metastasis Through Epithelial-to-Mesenchymal Transition Pathway.

Authors - Qifei Zou
  • KD Validated