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ASPH Antibody

ASPH Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14116-1-AP

Synonyms

AAH, ASP beta hydroxylase, aspartate beta hydroxylase, ASPH, BAH, CASQ2BP1, HAAH, JCTN, junctin



Tested Applications

Positive WB detected inhuman brain tissue, A549 cells
Positive IHC detected inhuman kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14116-1-AP targets ASPH in IHC, WB, ELISA applications and shows reactivity with human samples.

Reactivity human
Cited Species human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASPH fusion protein Ag5265
Full Name aspartate beta-hydroxylase
Calculated molecular weight 86 kDa
Observed molecular weight 26 kDa
GenBank accession number BC066929
Gene symbol ASPH
Gene ID (NCBI) 444
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ASPH antibody 14116-1-APDownload protocol
IHC protocol for ASPH antibody 14116-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Immunol Res

Construction and Characterization of Adenovirus Vectors Encoding Aspartate-β-Hydroxylase to Preliminary Application in Immunotherapy of Hepatocellular Carcinoma.

Authors - Yujiao Zhou
humanWB

EBioMedicine

Hydroxylase Activity of ASPH Promotes Hepatocellular Carcinoma Metastasis Through Epithelial-to-Mesenchymal Transition Pathway.

Authors - Qifei Zou
  • KD Validated