|Positive WB detected in||mouse brain tissue, A549 cells, human brain tissue|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14116-1-AP targets ASPH in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ASPH fusion protein Ag5265|
|Full Name||aspartate beta-hydroxylase|
|Calculated molecular weight||86 kDa|
|Observed molecular weight||26 kDa|
|GenBank accession number||BC066929|
|Gene ID (NCBI)||444|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Aspartate-β-hydroxylase (ASPH; termed AAH in older literature) has been implicated in the cross-talk among all of these signaling pathways. Correspondingly, ASPH is expressed at high levels in many malignant neoplasms of different histogeneses. It has the 86-141 kDa bands (native and phosphorylated forms), cleavage products of 35-56 and 22-26 kDa.(PMID:27981247/PMID: 10956665)
J Immunol Res
Construction and Characterization of Adenovirus Vectors Encoding Aspartate-β-Hydroxylase to Preliminary Application in Immunotherapy of Hepatocellular Carcinoma.
Hydroxylase Activity of ASPH Promotes Hepatocellular Carcinoma Metastasis Through Epithelial-to-Mesenchymal Transition Pathway.