ATG12 Polyclonal antibody

ATG12 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11122-1-AP

Synonyms

APG12, APG12 like, APG12L, ATG12, Autophagy related protein 12, FBR93, HAPG12, Ubiquitin like protein ATG12



Tested Applications

Positive WB detected inhuman bladder tissue, UV treated HEK-293
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11122-1-AP targets ATG12 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG12 fusion protein Ag1586
Full Name ATG12 autophagy related 12 homolog (S. cerevisiae)
Calculated molecular weight 16 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberBC011033
Gene symbol ATG12
Gene ID (NCBI) 9140
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.

Protocols

Product Specific Protocols
WB protocol for ATG12 antibody 11122-1-APDownload protocol
IHC protocol for ATG12 antibody 11122-1-APDownload protocol
IP protocol for ATG12 antibody 11122-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Mol Cancer

Long non-coding RNA GBCDRlnc1 induces chemoresistance of gallbladder cancer cells by activating autophagy.

Authors - Qiang Cai
humanIHC

Transl Androl Urol

miR-30a-3p inhibits renal cancer cell invasion and metastasis through targeting ATG12.

Authors - Yonghui Chen
mouseWB

Int J Clin Exp Med

Low doses of single or combined agrichemicals induces α-synuclein aggregation in nigrostriatal system of mice through inhibition of proteasomal and autophagic pathways.

Authors - Cen Su

Biomed Pharmacother

The gastric mucosal protective effects of astragaloside IV in mnng-induced GPL rats.

Authors - Tiantian Cai
mouseWB

J Endod

Anti-osteoclastogenesis of Mineral Trioxide Aggregate through Inhibition of the Autophagic Pathway.

Authors - Xue Cheng
humanWB

Chem Res Toxicol

Quinones Derived from Polychlorinated Biphenyls Induce ROS-Dependent Autophagy by Evoking an Autophagic Flux and Inhibition of mTOR/p70S6k.

Authors - Qiong Shi