ATG12 antibody (11264-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis using 11264-1-AP (same clone as 11264-1-PBS)
PC-3 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.
WB analysis using 11264-1-AP (same clone as 11264-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.
WB analysis of HCT 116 using 11264-1-AP (same clone as 11264-1-PBS)
HCT 116 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.
WB analysis of COLO 320 using 11264-1-AP (same clone as 11264-1-PBS)
COLO 320 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.
WB analysis of NIH/3T3 using 11264-1-AP (same clone as 11264-1-PBS)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 11264-1-AP (same clone as 11264-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 11264-1-AP (same clone as 11264-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 11264-1-AP (same clone as 11264-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 11264-1-AP (same clone as 11264-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.
IF Staining of PC-3 using 11264-1-AP (same clone as 11264-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ATG12 antibody (11264-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

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Tested Applications
Product Information

Product Information

11264-1-PBS targets ATG12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ATG12 fusion protein Ag1791 Predict reactive species
Full Name	ATG12 autophagy related 12 homolog (S. cerevisiae)
Calculated Molecular Weight	15 kDa
Observed Molecular Weight	48-55 kDa
GenBank Accession Number	BC012266
Gene Symbol	ATG12
Gene ID (NCBI)	9140
RRID	AB_10807096
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O94817
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.