|Positive WB detected in||Jurkat cells, THP-1 cells|
|Positive IP detected in||HT-1080 cells, NIH/3T3 cells|
|Positive IHC detected in||human cervical cancer tissue, mouse kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10088-2-AP targets ATG7 in WB, IP, IHC, IF applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, pig, prawn, rat, turtle|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATG7 fusion protein Ag0142|
|Full Name||ATG7 autophagy related 7 homolog (S. cerevisiae)|
|Calculated molecular weight||78 kDa|
|Observed molecular weight||68-78 kDa|
|GenBank accession number||BC000091|
|Gene ID (NCBI)||10533|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.
|Product Specific Protocols|
|WB protocol for ATG7 antibody 10088-2-AP||Download protocol|
|IHC protocol for ATG7 antibody 10088-2-AP||Download protocol|
|IF protocol for ATG7 antibody 10088-2-AP||Download protocol|
|IP protocol for ATG7 antibody 10088-2-AP||Download protocol|
|Click here to view our Standard Protocols|
Ecotoxicol Environ Saf
Evaluating expression of autophagy-related genes in oriental river prawn Macrobrachium nipponense as potential biomarkers for hypoxia exposure.
Reprod Biol Endocrinol
LIPOPHAGY: a novel form of steroidogenic activity within the LEYDIG cell during the reproductive cycle of turtle.
Cell Death Dis
Regulatory roles of miR-22/Redd1-mediated mitochondrial ROS and cellular autophagy in ionizing radiation-induced BMSC injury.
Biochem Biophys Res Commun
Autophagy exerts pivotal roles in regulatory effects of 1α,25-(OH)2D3 on the osteoclastogenesis.
In Vivo Autophagy Up-Regulation of Small Intestine Enterocytes in Chinese Soft-Shelled Turtles during Hibernation.
Br J Oral Maxillofac Surg
Increased expression of autophagy-related proteins in keratocystic odontogenic tumours: its possible association with growth potential.