Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, mouse liver tissue, THP-1 cells, U2OS cells |
| Positive IP detected in | HT-1080 cells, NIH/3T3 cells |
| Positive IHC detected in | human cervical cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10088-2-AP targets ATG7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, canine, chicken, hamster, goat, turtle |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0142 Product name: Recombinant human ATG7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 293-563 aa of BC000091 Sequence: AFSPDCPKAVGWEKNQKGGMGPRMVNLSECMDPKRLAESSVDLNLKLMCWRLVPTLDLDKVVSVKCLLLGAGTLGCNVARTLMGWGVRHITFVDNAKISYSNPVRQPLYEFEDCLGGGKPKALAAADRLQKIFPGVNARGFNMSIPMPGHPVNFSSVTLEQARRDVEQLEQLIESHDVVFLLMDTRESRWLPAVIAASKRKLVINAALGFDTFVVMRHGLKKPKQQGAGDLCPNHPVASADLLGSSLFANIPGYKLGCYFCNDVVAPGDST Predict reactive species |
| Full Name | ATG7 autophagy related 7 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 78 kDa |
| Observed Molecular Weight | 68-78 kDa |
| GenBank Accession Number | BC000091 |
| Gene Symbol | ATG7 |
| Gene ID (NCBI) | 10533 |
| RRID | AB_2062351 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95352 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATG7 antibody 10088-2-AP | Download protocol |
| IHC protocol for ATG7 antibody 10088-2-AP | Download protocol |
| IP protocol for ATG7 antibody 10088-2-AP | Download protocol |
| WB protocol for ATG7 antibody 10088-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ATG7 Polyclonal antibody (Cat# 10088-2-AP) has 230 total citations. Publications appear in high-impact journals including Nature, Nature Cell Biology, Nature Communications, Autophagy, Cell Death & Differentiation, Cell Death & Disease, Advanced Science, Molecular Cell, and PNAS. Research fields span autophagy and mitophagy, cancer biology, ferroptosis, immunology, neurology, cardiovascular disease, liver pathology, metabolic disorders, viral infections, bone/joint repair, and environmental toxicology. Applications are predominantly Western blot, with additional use in IF, IHC, and IP.
| Species | Application | Title |
|---|---|---|
Bone Res Chaperone-mediated autophagy directs a dual mechanism to balance premature senescence and senolysis to prevent intervertebral disc degeneration | ||
Bone Res SphK1/mitophagy axis in cementocytes drives orthodontic root resorption via mitochondrial transfer to osteoclasts. | ||
Autophagy AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability.
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Reviews
What customers say
Most reviewers (4 out of 5) rated this antibody 5 stars for Western Blot, using it at 1:1000–1:2000 in Caco-2 cells, mouse colon, and mouse liver tissue. Users reported a clear ATG7 band, with one noting it performed better than alternatives from other companies. The single 1-star review described a non-specific band in HeLa cells treated with ATG7-targeting siRNA, where expected downregulation was not clearly visible. Overall, the antibody is well received for WB in intestinal and hepatic samples, though results may vary by cell type.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Margaux (Verified Customer) (06-20-2025) | Used at 1/1000 in HeLa cells treated or not with siRNA targeting ATG7. I noticed non-specific band and couldn't see the downregulation of ATG7 protein expression in the siRNA condition.
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Priya (Verified Customer) (10-03-2024) | Used this antibody for colon carcinoma cell line and mice tissue
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Priya (Verified Customer) (08-09-2023) | Used for Caco2 cells and mice colon tissue
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Kamal (Verified Customer) (06-29-2023) | Atg7 band showed up better than from other companies.
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Priya (Verified Customer) (06-21-2023) | Used this antibody for Caco2 cells and mice tissue
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