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- KD/KO Validated
ATG7 Polyclonal antibody
ATG7 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse and More (5)
WB, IP, IHC, IF, ELISA
Cat no : 10088-2-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, mouse liver tissue, THP-1 cells, U2OS cells|
|Positive IP detected in||HT-1080 cells, NIH/3T3 cells|
|Positive IHC detected in||human cervical cancer tissue, mouse kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 14 publications below|
|WB||See 81 publications below|
|IHC||See 12 publications below|
|IF||See 6 publications below|
10088-2-AP targets ATG7 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, goat, pig, prawn, turtle|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATG7 fusion protein Ag0142|
|Full Name||ATG7 autophagy related 7 homolog (S. cerevisiae)|
|Calculated molecular weight||78 kDa|
|Observed molecular weight||68-78 kDa|
|GenBank accession number||BC000091|
|Gene ID (NCBI)||10533|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.
|Product Specific Protocols|
|WB protocol for ATG7 antibody 10088-2-AP||Download protocol|
|IHC protocol for ATG7 antibody 10088-2-AP||Download protocol|
|IF protocol for ATG7 antibody 10088-2-AP||Download protocol|
|IP protocol for ATG7 antibody 10088-2-AP||Download protocol|
|Click here to view our Standard Protocols|
Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.
Cell Death Differ
STING directly activates autophagy to tune the innate immune response.
Autophagy regulates functional differentiation of mammary epithelial cells.
Negative pressure wound therapy improves bone regeneration by promoting osteogenic differentiation via the AMPK-ULK1-autophagy axis.
AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability.
Proc Natl Acad Sci U S A
Mitochondrial Nuclear Retrograde Regulator 1 (MNRR1) rescues the cellular phenotype of MELAS by inducing homeostatic mechanisms.