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  • KD/KO Validated

ATG7 Polyclonal antibody

ATG7 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (7)





Cat no : 10088-2-AP


APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, GSA7, hAGP7

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, mouse liver tissue, THP-1 cells, U2OS cells
Positive IP detected inHT-1080 cells, NIH/3T3 cells
Positive IHC detected inhuman cervical cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10088-2-AP targets ATG7 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, turtle, goat, chicken, hamster, pig, prawn
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG7 fusion protein Ag0142
Full Name ATG7 autophagy related 7 homolog (S. cerevisiae)
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 68-78 kDa
GenBank Accession NumberBC000091
Gene Symbol ATG7
Gene ID (NCBI) 10533
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.


Product Specific Protocols
WB protocol for ATG7 antibody 10088-2-APDownload protocol
IHC protocol for ATG7 antibody 10088-2-APDownload protocol
IF protocol for ATG7 antibody 10088-2-APDownload protocol
IP protocol for ATG7 antibody 10088-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Adv Sci (Weinh)

Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy

Authors - Qian Gou

Nat Commun

Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.

Authors - Jiawei Zhang
  • KD Validated

Cell Death Differ

STING directly activates autophagy to tune the innate immune response.

Authors - Dong Liu
mouseWB, IHC


Autophagy regulates functional differentiation of mammary epithelial cells.

Authors - Jessica Elswood


Negative pressure wound therapy improves bone regeneration by promoting osteogenic differentiation via the AMPK-ULK1-autophagy axis.

Authors - Sheng Zhang


AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability.

Authors - Ashwinkumar Subramenium Ganapathy
  • KO Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Priya (Verified Customer) (08-09-2023)

Used for Caco2 cells and mice colon tissue

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 to 1000
  • Cell Tissue Type: Caco2, Mice colon

Kamal (Verified Customer) (06-29-2023)

Atg7 band showed up better than from other companies.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse Liver tissue
ATG7 Antibody Western Blot validation (1:2000 dilution) in Mouse Liver tissue (Cat no:10088-2-AP)

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells and mice tissue

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 to 1000
  • Cell Tissue Type: Caco2, Mice colon