ATM Monoclonal antibody

ATM Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3E6F4

Cat no : 67586-1-Ig

Synonyms

A T mutated, AT1, ATA, ataxia telangiectasia mutated, ATC, ATD, ATDC, ATE, ATM, DKFZp781A0353, Serine protein kinase ATM, TEL1, TELO1



Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

67586-1-Ig targets ATM in WB, IF, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Cited Reactivityhuman, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATM fusion protein Ag30001
Full Name ataxia telangiectasia mutated
Observed molecular weight 310-350 kDa
GenBank accession numberBC137169
Gene symbol ATM
Gene ID (NCBI) 472
RRIDAB_2882795
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is involved in mitogenic signal transduction, meiotic recombination, detection of DNA damage, and cell cycle control.

Protocols

Product Specific Protocols
WB protocol for ATM antibody 67586-1-IgDownload protocol
IF protocol for ATM antibody 67586-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Food Chem Toxicol

DON entry into the nucleus induces DNA damage, apoptosis and cycle arrest in GES-1 cells

Authors - Silu Hou
ratIF,WB

CNS Neurosci Ther

Multiple sevoflurane exposures during mid-trimester induce neurotoxicity in the developing brain initiated by 15LO2-Mediated ferroptosis

Authors - Qian Jiang