ATP5J2 Polyclonal antibody

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  WB analysis using 15842-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15842-1-AP (ATP5J2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 15842-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ATP5J2 antibody (15842-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

ATP5J2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 15842-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

ATP synthase membrane subunit f, ATP5JL, ATP5MF

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15842-1-AP targets ATP5J2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8598

  Product name: Recombinant human ATP5J2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-94 aa of BC003678

  Sequence: MASVGECPAPVPVKDKKLLEVKLLELPSWILMRDFSPSGIFGAFQRGYYRYYNKYINVKKGSISGITMVLACYVLFSYSFSYKHLKHERLRKYH

• Full Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F2
• Calculated Molecular Weight: 5-11 kDa
• Observed Molecular Weight: 11kDa
• GenBank Accession Number: BC003678
• Gene Symbol: ATP5J2
• Gene ID (NCBI): 9551
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P56134
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP5J2 (also known as ATP synthase-coupling factor 6, or CF6) is a nuclear-encoded subunit of mitochondrial ATP synthase (Complex V), the enzyme responsible for synthesizing ATP from ADP and inorganic phosphate (Pi) during oxidative phosphorylation (OXPHOS).