ATP7A-Specific Polyclonal antibody

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  WB analysis using 55375-1-AP

  Unboiled mouse lung tissue were subjected to SDS PAGE followed by western blot with 55375-1-AP (ATP7A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 55375-1-AP

  IP result of anti-ATP7A-Specific (IP:55375-1-AP, 4ug; Detection:55375-1-AP 1:500) with mouse lung tissue lysate 1520 ug.

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  IHC staining of human lung cancer using 55375-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55375-1-AP (ATP7A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATP7A-Specific Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 55375-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

ATP7A, ATPase, Cu++ transporting, alpha polypeptide, Copper transporting ATPase 1

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Tested Applications

• Positive WB detected in: unboiled mouse lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55375-1-AP targets ATP7A-Specific in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: ATPase, Cu++ transporting, alpha polypeptide
• Calculated Molecular Weight: 165 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: NM_000052
• Gene Symbol: ATP7A
• Gene ID (NCBI): 538
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q04656
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATPase copper transporting alpha(ATP7A) is a type of transmembrane P-type ATPase for copper transport. ATP7A and ATP7B are homologous genes responsible for copper transport across cell membranes. ATP7A transports copper ions from the Golgi membrane to copper-dependent enzymes (such as dopamine β-hydroxylase) in the secretory pathway. When copper ion concentrations rise, ATP7A relocalizes from the Golgi apparatus to the plasma membrane, promoting copper efflux and thereby protecting cells from copper toxicity. Mutations in ATP7A are associated with Menkes disease (MNK)( PMID: 28389643, 30341172).