ATPAF2 Polyclonal antibody

ATPAF2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11598-1-AP

Synonyms

ATP12, ATP12 homolog, ATP12p, ATPAF2, LP3663

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Validation Data Gallery

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  WB analysis of HEK-293 using 11598-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11598-1-AP (ATPAF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11598-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11598-1-AP (ATPAF2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse heart using 11598-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11598-1-AP (ATPAF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 11598-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11598-1-AP (ATPAF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11598-1-AP targets ATPAF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATPAF2 fusion protein Ag2164
• Full Name: ATP synthase mitochondrial F1 complex assembly factor 2
• Calculated Molecular Weight: 289 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC032126
• Gene Symbol: ATPAF2
• Gene ID (NCBI): 91647
• RRID: AB_2063032
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Neurobiol Aging; The perimenopausal aging transition in the female rat brain: decline in bioenergetic systems and synaptic plasticity.; Authors - Fei Yin