Ataxin-1 Polyclonal antibody

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  WB analysis using 27265-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27265-1-AP (Ataxin-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Ataxin-1 Polyclonal Antibody for WB, ELISA

Cat No. 27265-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ATXN1, ataxin 1, ataxin1, ATX1, ATXN 1

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27265-1-AP targets Ataxin-1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26099

  Product name: Recombinant human ATXN1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 301-400 aa of NM_000332

  Sequence: VPREATKKAESSRLQQAIQAKEVLNGEMEKSRRYGAPSSADLGLGKAGGKSVPHPYESRHVVVHPSPSDYSSRDPSGVRASVMVLPNSNTPAADLEVQQA

• Full Name: ataxin 1
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: NM_000332
• Gene Symbol: ATXN1
• Gene ID (NCBI): 6310
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P54253
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ataxin-1 (ATXN1) is a protein involved in transcriptional regulation and RNA binding. It is a transcriptional repressor that interacts with multiple transcription factors and is associated with large protein complexes. ATXN1 is also involved in the regulation of alternative splicing, as demonstrated by its ability to regulate the splicing of several minigenes. The protein is crucial for normal cerebellar function and is implicated in the pathogenesis of spinocerebellar ataxia type 1 (SCA1), a neurodegenerative disorder caused by the expansion of a polyglutamine tract in ATXN1 (PMID: 21475249; 20628574).