AXIN1 Monoclonal antibody
AXIN1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1C4E8
Cat no : 68093-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, HSC-T6 cells, NIH/3T3 cells, MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Sample-dependent, check data in validation data gallery |
Product Information
68093-1-Ig targets AXIN1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | AXIN1 fusion protein Ag10079 |
Full Name | axin 1 |
Calculated molecular weight | 826aa,92 kDa; 862aa,95 kDa |
Observed molecular weight | 110-120 kDa |
GenBank accession number | BC044648 |
Gene symbol | AXIN1 |
Gene ID (NCBI) | 8312 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Axis inhibition protein1 (AXIN1), also called AXIN, together with AXIN2 are multidomain scaffold proteins that negatively regulate Wnt signaling. AXIN1 is likely to function as a tumor suppressor. Under UV irradiation, AXIN1-HIPK2-TP53 complex forms. The complex also controls cell growth, apoptosis and development. Like AXIN2, AXIN1 undergoes poly(ADP-ribosy)lation by tankyrase TNKS and TNKS2 followed by unbiquitination by RNF146 which leads to its degradation and subsequent activation of Wnt signaling. Its deubiquitination by USP34 is important for nuclear accumulation during Wnt signaling. Proteintech's AXIN1 antibody 16541-1-AP is a rabbit polyclonal antibdy raised against the N-terminus of human AXIN1.
Protocols
Product Specific Protocols | |
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WB protocol for AXIN1 antibody 68093-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |