Acetyl-Histone H4 (Lys12) Recombinant monoclonal antibody, PBS Only

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  WB analysis of NIH/3T3 using 83095-1-RR (same clone as 83095-1-PBS)

  Untreated and trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 83095-1-RR (same clone as 83095-1-PBS)

  WB result of Acetyl-Histone H4 (Lys12) antibody (83095-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Acetyl-Histone H4 (Lys12) transfected HEK-293 cells. This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 83095-1-RR (same clone as 83095-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 83095-1-RR (same clone as 83095-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 83095-1-RR (same clone as 83095-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 83095-1-RR (same clone as 83095-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 83095-1-RR (Acetyl-Histone H4 (Lys12) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 83095-1-RR (same clone as 83095-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Acetyl-Histone H4 (Lys12) antibody (83095-1-RR, Clone: 1D18 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 83095-1-RR (same clone as 83095-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Acetyl-Histone H4 (Lys12) antibody (83095-1-RR, Clone: 1D18 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

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  Dot Blot experiment of peptide using 83095-1-RR (same clone as 83095-1-PBS)

  Dot blot analysis was used to confirm the specificity of Histone H4AK12ac antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. Column 1: H4AK12ac. Column 2: Unmodified H4AK12. Column 3: H4AK5ac. Column 4: Unmodified H4AK5. Column 5: H4AK8Ac. Column 6: Unmodified H4AK8. Column 7: H4AK16ac. Column 8:Unmodified H4AK16. Column 9: H4AK91ac. Column 10: Unmodified H4K91 Column 11: Blank(PBS). This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

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  ChIP experiment of HeLa using 83095-1-RR (same clone as 83095-1-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H4 (Lys12) (83095-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 83095-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Acetyl-Histone H4 (Lys12) Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Cat No. 83095-1-PBS

Clone No.1D18

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Synonyms

Ac Histone H4, Ac Histone H4 (Lys12), Ac Histone H4 Lys12, Ac Histone H4-Lys12, Acetyl Histone

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Product Information

83095-1-PBS targets Acetyl-Histone H4 (Lys12) in WB, IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 1, H4a
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC069654
• Gene Symbol: HIST1H4A
• Gene ID (NCBI): 8359
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62805
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Acetylation of histone H4 is necessary for chromatin decompaction during DNA replication.