Tested Applications
| Positive WB detected in | Trichostatin A treated NIH/3T3 cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | mouse small intestine tissue, human placenta tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Caco-2 cells, Trichostatin A treated NIH/3T3 cells |
| Positive Dot Blot detected in | peptide |
| Positive ChIP-qPCR detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| DOT BLOT | DOT BLOT : 1:10-1:100 |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83095-1-RR targets Acetyl-Histone H4 (Lys12) in WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | histone cluster 1, H4a |
| Observed Molecular Weight | 12 kDa |
| GenBank Accession Number | BC069654 |
| Gene Symbol | HIST1H4A |
| Gene ID (NCBI) | 8359 |
| RRID | AB_3670809 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P62805 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Acetylation of histone H4 is necessary for chromatin decompaction during DNA replication.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Acetyl-Histone H4 (Lys12) antibody 83095-1-RR | Download protocol |
| IHC protocol for Acetyl-Histone H4 (Lys12) antibody 83095-1-RR | Download protocol |
| WB protocol for Acetyl-Histone H4 (Lys12) antibody 83095-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Acetyl-Histone H4 (Lys12) Recombinant monoclonal antibody (1D18), catalog number 83095-1-RR, has 3 total citations. These appear in Advanced Science, International Journal of Biological Macromolecules, and Cell Signaling. The research fields span histone modification and colitis, epithelial-mesenchymal transition in colorectal cancer, and oral squamous cell carcinoma progression with antitumor immunity. Applications cited include WB and ChIP.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) ACSS2-Mediated Histone H4 Lysine 12 Crotonylation (H4K12cr) Alleviates Colitis via Enhancing Transcription of CLDN7 | ||
Int J Biol Macromol Bone marrow tyrosine kinase on chromosome X promotes epithelial-mesenchymal transition through signal transducer and activator of transcription 3 in colorectal cancer. | ||
Cell Signal N-α-Acetyltransferase 40 promotes oral squamous cell carcinoma progression by enhancing FEN1 transcription and suppressing CD8(+) T cell antitumor immunity. |
Reviews
What customers say
Both reviewers gave 5-star ratings for Western Blot applications. One user tested the antibody at 1:1000 dilution on MCF10A cells to evaluate H4K12ac levels in mammalian cells, reporting it worked well and highly recommending it. The other user used a 1:10,000 dilution on primary HMECs with no additional comments. Both reviews reference the same lot (23007031). Overall, the antibody is well-received for WB with strong performance across different cell types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Li (Verified Customer) (10-08-2026) |
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Sree Rama Chaitanya (Verified Customer) (07-21-2025) | I have tested this antibody to evaluate H4K12ac levels in mammalian cells, and it works well for WB. Highly recommend!
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