acetylated Tubulin(Lys40) Monoclonal antibody
acetylated Tubulin(Lys40) Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, Canine, pig and More (3)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
7E5H8
Cat no : 66200-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | rat testis tissue, NCCIT cells, Neuro-2a cells, L02 cells, pig cerebellum tissue, rat brain tissue, mouse ovary tissue, HEK-293 cells, mouse testis |
| Positive IHC detected in | mouse ovary tissue, human gliomas tissue, rat brain tissue, mouse brain tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MDCK cells, mouse brain tissue, islet |
| Positive ELISA detected in | Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 39 publications below |
| IHC | See 2 publications below |
| IF | See 35 publications below |
Product Information
66200-1-Ig targets acetylated Tubulin(Lys40) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, Canine, pig samples.
| Tested Reactivity | human, mouse, rat, Canine, pig |
| Cited Reactivity | human, mouse, rat, bovine, Drosophila, pig, zebrafish |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | tubulin, alpha 1a |
| Calculated molecular weight | 52 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | NM_006009 |
| Gene symbol | TUBA1A |
| Gene ID (NCBI) | 7846 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
What is the molecular weight of acetyl-α-tubulin?
The molecular weight of acetylated tubulin is 52 kD.
Where does the acetylation of Lys 40 occur?
This acetylation occurs inside the microtubule lumen by the α-tubulin acetyltransferase 1 (αTAT1) (PMID: 29207274).
How can acetylation be reversed?
Acetylation of Lys 40 can be reversed by deacetylase 6 (HDAC6), which is mostly cytoplasmic that also deacetylates Hsp90, and sirtuin 2 (SIRT2), which also mainly cytoplasmic and uses NAD as a coenzyme. Unlike HDAC6, SIRT uses both polymerized and soluble tubulin as substrates. Deacetylases are believed to be more active on soluble tubulin, while acetylases function preferentially on stable polymers (PMIDs: 29207274, 30079247, 19185337).
What is the function of acetylation?
Acetylation is a conserved post-translational modification of alpha tubulin at Lys 40 during tubulin assembly, and it correlates to increased microtubule stability and intracellular transport (PMIDs: 29207274, 30079247, 20940043).
Is acetylation of α-tubulin strictly associated with stable microtubules?
Not necessarily, as acetylation can have other effects on microtubule subpopulations (PMID 20940043).
Is ac-tubulin found only in cilia?
Acetylated-α-tubulin is located in cytoplasmic tubulin as well as in cilia; therefore, it is not strictly region-specific (PMID: 30079247).
What are the cellular effects of tubulin acetylation?
Microtubule acetylation seems to provide a critical role in neuronal development and function, and while its effect on cancer cells remains unclear, it has been shown that decreased acetylated α-tubulin impairs neuronal cell line migration. The post-translational modification may also help regulate organelle-independent signalling throughout the cell, supporting the notion of a microtubule network serving as a coordinator of cellular signaling (PMIDs: 29207274, 25503560, 20940043, 19185337).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for acetylated Tubulin(Lys40) antibody 66200-1-Ig | Download protocol |
| IHC protocol for acetylated Tubulin(Lys40) antibody 66200-1-Ig | Download protocol |
| IF protocol for acetylated Tubulin(Lys40) antibody 66200-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Invest Nasal ciliated cells are primary targets for SARS-CoV-2 replication in early stage of COVID-19. | ||
Int J Biol Sci NLRP3 Inflammasome Mediates Silica-induced Lung Epithelial Injury and Aberrant Regeneration in Lung Stem/Progenitor Cell-derived Organotypic Models | ||
Biomaterials A virus-induced kidney disease model based on organ-on-a-chip: Pathogenesis exploration of virus-related renal dysfunctions. | ||
Dev Cell HDAC6 Suppresses Age-Dependent Ectopic Fat Accumulation by Maintaining the Proteostasis of PLIN2 in Drosophila. | ||
Am J Hum Genet C11orf70 Mutations Disrupting the Intraflagellar Transport-Dependent Assembly of Multiple Axonemal Dyneins Cause Primary Ciliary Dyskinesia. | ||
Theranostics The histone deacetylase inhibitor PCI-24781 impairs calcium influx and inhibits proliferation and metastasis in breast cancer. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Mi (Verified Customer) (05-29-2023) | Excellent antibody, it works pretty well in human white adipocytes!
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FH Sonia (Verified Customer) (04-08-2022) | Very good specificity and sensitivity. I used for IHC in different paraffin-embedded cell blocks for different cell lines, previously analyzed by WB.
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