acetylated Tubulin(Lys40) Monoclonal antibody
acetylated Tubulin(Lys40) Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
human, mouse, rat, Canine, pig and More (3)
WB, IHC, IF, ELISA
Cat no : 66200-1-Ig
Validation Data Gallery
|Positive WB detected in||rat testis tissue, NCCIT cells, Neuro-2a cells, L02 cells, pig cerebellum tissue, rat brain tissue, mouse ovary tissue, HEK-293 cells, mouse testis|
|Positive IHC detected in||mouse ovary tissue, human gliomas tissue, rat brain tissue, mouse brain tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MDCK cells, mouse brain tissue, islet|
|Positive ELISA detected in||Recombinant protein|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Enzyme-linked Immunosorbent Assay (ELISA)||ELISA : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
|WB||See 39 publications below|
|IHC||See 2 publications below|
|IF||See 35 publications below|
66200-1-Ig targets acetylated Tubulin(Lys40) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, Canine, pig samples.
|Tested Reactivity||human, mouse, rat, Canine, pig|
|Cited Reactivity||human, mouse, rat, bovine, Drosophila, pig, zebrafish|
|Host / Isotype||Mouse / IgG1|
|Full Name||tubulin, alpha 1a|
|Calculated molecular weight||52 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||NM_006009|
|Gene ID (NCBI)||7846|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
What is the molecular weight of acetyl-α-tubulin?
The molecular weight of acetylated tubulin is 52 kD.
Where does the acetylation of Lys 40 occur?
This acetylation occurs inside the microtubule lumen by the α-tubulin acetyltransferase 1 (αTAT1) (PMID: 29207274).
How can acetylation be reversed?
Acetylation of Lys 40 can be reversed by deacetylase 6 (HDAC6), which is mostly cytoplasmic that also deacetylates Hsp90, and sirtuin 2 (SIRT2), which also mainly cytoplasmic and uses NAD as a coenzyme. Unlike HDAC6, SIRT uses both polymerized and soluble tubulin as substrates. Deacetylases are believed to be more active on soluble tubulin, while acetylases function preferentially on stable polymers (PMIDs: 29207274, 30079247, 19185337).
What is the function of acetylation?
Acetylation is a conserved post-translational modification of alpha tubulin at Lys 40 during tubulin assembly, and it correlates to increased microtubule stability and intracellular transport (PMIDs: 29207274, 30079247, 20940043).
Is acetylation of α-tubulin strictly associated with stable microtubules?
Not necessarily, as acetylation can have other effects on microtubule subpopulations (PMID 20940043).
Is ac-tubulin found only in cilia?
Acetylated-α-tubulin is located in cytoplasmic tubulin as well as in cilia; therefore, it is not strictly region-specific (PMID: 30079247).
What are the cellular effects of tubulin acetylation?
Microtubule acetylation seems to provide a critical role in neuronal development and function, and while its effect on cancer cells remains unclear, it has been shown that decreased acetylated α-tubulin impairs neuronal cell line migration. The post-translational modification may also help regulate organelle-independent signalling throughout the cell, supporting the notion of a microtubule network serving as a coordinator of cellular signaling (PMIDs: 29207274, 25503560, 20940043, 19185337).
|Product Specific Protocols|
|WB protocol for acetylated Tubulin(Lys40) antibody 66200-1-Ig||Download protocol|
|IHC protocol for acetylated Tubulin(Lys40) antibody 66200-1-Ig||Download protocol|
|IF protocol for acetylated Tubulin(Lys40) antibody 66200-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
J Clin Invest
Nasal ciliated cells are primary targets for SARS-CoV-2 replication in early stage of COVID-19.
Int J Biol Sci
NLRP3 Inflammasome Mediates Silica-induced Lung Epithelial Injury and Aberrant Regeneration in Lung Stem/Progenitor Cell-derived Organotypic Models
A virus-induced kidney disease model based on organ-on-a-chip: Pathogenesis exploration of virus-related renal dysfunctions.
HDAC6 Suppresses Age-Dependent Ectopic Fat Accumulation by Maintaining the Proteostasis of PLIN2 in Drosophila.
Am J Hum Genet
C11orf70 Mutations Disrupting the Intraflagellar Transport-Dependent Assembly of Multiple Axonemal Dyneins Cause Primary Ciliary Dyskinesia.
The histone deacetylase inhibitor PCI-24781 impairs calcium influx and inhibits proliferation and metastasis in breast cancer.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Mi (Verified Customer) (05-29-2023)
Excellent antibody, it works pretty well in human white adipocytes!
Sonia (Verified Customer) (04-08-2022)
Very good specificity and sensitivity. I used for IHC in different paraffin-embedded cell blocks for different cell lines, previously analyzed by WB.