CoraLite® Plus 488-conjugated Acetyl-Tubulin (Lys40) Monoclonal antibody

Acetyl-Tubulin (Lys40) Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, Canine, pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

7E5H8

Cat no : CL488-66200

Synonyms

Ac tubulin, acetylated alpha tubulin, Acetylated tubulin, acetylated Tubulin (Lys40), acetylated Tubulin(Lys40), acetylated-a-tubulin, acetyl-a-tubulin, acetyl-tubulin, Ac-tubulin, alpha tubulin, Alpha tubulin 3, TUBA1A, TUBA1A(ace-40Lys), TUBA3, tubulin, Tubulin alpha 3 chain, Tubulin B alpha 1, tubulin, alpha 1a



Tested Applications

Positive IF detected inMDCK cells, mouse kidney tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 3 publications below

Product Information

CL488-66200 targets Acetyl-Tubulin (Lys40) in IF applications and shows reactivity with human, mouse, rat, Canine, pig samples.

Tested Reactivity human, mouse, rat, Canine, pig
Cited Reactivityhuman, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name tubulin, alpha 1a
Calculated Molecular Weight 52 kDa
GenBank Accession NumberNM_006009
Gene Symbol Alpha Tubulin
Gene ID (NCBI) 7846
RRIDAB_2883282
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation/deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin; it does not recognize non-acetylated tubulin. (24268707, 23881549). This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Acetyl-Tubulin (Lys40) antibody CL488-66200Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Front Cell Dev Biol

PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia

Authors - Tingting Zhang
humanIF

STAR Protoc

Isolation, expansion, differentiation, and histological processing of human nasal epithelial cells

Authors - Vincent Manna
humanIF

BBA Adv

The dynamic nature of the coronavirus receptor, angiotensin-converting enzyme 2 (ACE2) in differentiating airway epithelia.

Authors - Vincent J Manna