CoraLite® Plus 488-conjugated acetylated Tubulin(Lys40) Monoclonal antibody
acetylated Tubulin(Lys40) Monoclonal Antibody for IF
Cat no : CL488-66200
|Positive IF detected in||MDCK cells, mouse kidney tissue|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
CL488-66200 targets acetylated Tubulin(Lys40) in IF applications and shows reactivity with human, mouse, rat, Canine, pig samples.
|Tested Reactivity||human, mouse, rat, Canine, pig|
|Cited Reactivity||human, rat|
|Host / Isotype||Mouse / IgG1|
|Full Name||tubulin, alpha 1a|
|Calculated molecular weight||52 kDa|
|GenBank accession number||NM_006009|
|Gene ID (NCBI)||7846|
|Conjugate||CoraLite® Plus 488 Fluorescent Dye|
|Excitation/Emission maxima wavelengths||493 nm / 522 nm|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.|
|Storage Conditions||Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation/deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin; it does not recognize non-acetylated tubulin. (24268707, 23881549). This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
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