CL594-conjugated acetylated Tubulin(Lys40) Antibody

acetylated Tubulin(Lys40) Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, dog, pig

Applications

IF, ELISA

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

7E5H8

Cat no : CL594-66200

Synonyms

alpha tubulin, Alpha tubulin 3, B ALPHA 1, LIS3, TUBA1A, TUBA3, Tubulin alpha 1A chain, Tubulin alpha 3 chain, Tubulin B alpha 1, tubulin, alpha 1a



Tested Applications

Positive IF detected inMDCK cells
For other applications, we recommend the unconjugated version of this antibody, 66200-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66200 targets CL594-conjugated acetylated Tubulin(Lys40) in IF, ELISA applications and shows reactivity with human, mouse, rat, dog, pig samples.

Tested Reactivity human, mouse, rat, dog, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name tubulin, alpha 1a
Calculated molecular weight 52 kDa
Observed molecular weight
GenBank accession number NM_006009
Gene symbol TUBA1A
Gene ID (NCBI) 7846
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation/deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin; it does not recognize non-acetylated tubulin. (24268707, 23881549) And this anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Protocols

Product Specific Protocols
IF protocol for CL594-conjugated acetylated Tubulin(Lys40) antibody CL594-66200Download protocol
Standard Protocols
Click here to view our Standard Protocols