Agrin Recombinant monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA sample test of MP02476-2

  The mean AGRN concentration was determined to be 5.3 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load and 3.4 ng/mL in SH-SY5Y cell extract based on a 1.2 mg/mL extract load.

• 

  Sandwich ELISA sample test of MP02476-2

  Plasma of six individual healthy human donors was measured. The AGRN concentration of detected samples was determined to be 11.4 ng/mL with a range of 5.1-28.2 ng/mL.

• 

  Sandwich ELISA standard curve of MP02476-2

  Sandwich ELISA standard curve of MP02476-2, Human AGRN Recombinant Matched Antibody Pair - PBS only. 86507-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg5123. 86507-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

Agrin Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Cat No. 86507-5-PBS

Clone No.251295A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA

Synonyms

AGRN, Agrin C-terminal 110 kDa subunit, Agrin C-terminal 22 kDa fragment, Agrin C-terminal 90 kDa fragment, Agrin N-terminal 110 kDa subunit

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Product Information

86507-5-PBS targets Agrin as part of a matched antibody pair:

MP02476-2: 86507-5-PBS capture and 86507-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5123

  Product name: recombinant human Agrin protein

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 1260-2045 aa of NM_198576.4

  Sequence: ATSGAIAAGATARATTASRLPSSAVTPRAPHPSHTSQPVAKTTAAPTTRRPPTTAPSRVPGRRPPAPQQPPKPCDSQPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPCQPNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPVPHTVLNLKEPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTP

• Full Name: agrin
• Calculated Molecular Weight: 215 kDa
• GenBank Accession Number: NM_198576.4
• Gene Symbol: AGRN
• Gene ID (NCBI): 375790
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00468-6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.