Albumin Recombinant antibody

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  WB analysis using 83289-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83289-4-RR (Albumin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83289-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83289-4-RR against Mouse Albumin were performed. The affinity constant is 0.883 nM.

Albumin {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 83289-4-RR

Clone No.240245E1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Alb, Alb 1, Alb1, albumin

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Tested Applications

• Positive WB detected in: mouse retina tissue, mouse liver tissue, mouse cerebellum

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83289-4-RR targets Albumin in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: albumin
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: NM_009654.4
• Gene Symbol: Albumin
• Gene ID (NCBI): 11657
• RRID: AB_3670956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P07724
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. In additon, glycated serum albumin is also a potential diabetes biomarker. The N-terminal 24aa of albumin will be cleaved to generate the mature form of serum albumin with MW 66 kDa. (21388516, 23832071)