Aldolase C Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 66120-1-Ig (same clone as 66120-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66120-1-Ig (same clone as 66120-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66120-1-Ig (same clone as 66120-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66120-1-Ig (Aldolase C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66120-1-Ig (same clone as 66120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66120-1-Ig (same clone as 66120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66120-1-Ig (same clone as 66120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66120-1-Ig (same clone as 66120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66120-1-Ig (same clone as 66120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66120-1-Ig (same clone as 66120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66120-1-Ig (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66120-1-Ig (same clone as 66120-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Aldolase C antibody (66120-1-Ig, Clone: 5H7A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66120-1-Ig (same clone as 66120-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Aldolase C antibody (66120-1-Ig, Clone: 5H7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66120-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51427-1

  Cytometric bead array sample test of MP51427-1, ALDOC Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66120-1-PBS. Detection antibody: 66120-2-PBS.

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  Cytometric bead array standard curve of MP51427-1

  Cytometric bead array standard curve of MP51427-1, ALDOC Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66120-1-PBS. Detection antibody: 66120-2-PBS. Standard:Ag6779. Range: 0.781-100 ng/mL.

Aldolase C Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 66120-1-PBS

Clone No.5H7A5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

ALDOC, 5H7A5, ALDC, ALDO, Brain-type aldolase

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Product Information

66120-1-PBS targets Aldolase C as part of a matched antibody pair:

MP51427-1: 66120-1-PBS capture and 66120-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6779

  Product name: Recombinant human ALDOC protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-364 aa of BC003613

  Sequence: MPHSYPALSAEQKKELSDIALRIVAPGKGILAADESVGSMAKRLSQIGVENTEENRRLYRQVLFSADDRVKKCIGGVIFFHETLYQKDDNGVPFVRTIQDKGIVVGIKVDKGVVPLAGTDGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKISERTPSALAILENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHVYLEGTLLKPNMVTPGHACPIKYTPEEIAMATVTALRRTVPPAVPGVTFLSGGQSEEEASFNLNAINRCPLPRPWALTFSYGRALQASALNAWRGQRDNAGAATEEFIKRAEVNGLAAQGKYEGSGEDGGAAAQSLYIANHAY

• Full Name: aldolase C, fructose-bisphosphate
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC003613
• Gene Symbol: Aldolase C
• Gene ID (NCBI): 230
• RRID: AB_2881519
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P09972
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involed in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).