Aldolase C Monoclonal antibody

Aldolase C Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5H7A5

Cat no : 66120-1-Ig

Synonyms

ALDOC, Aldolase C, Brain type aldolase, Fructose bisphosphate aldolase C



Tested Applications

Positive WB detected inHeLa cells, human brain tissue, human heart tissue, HepG2 cells, K-562 cells, HSC-T6 cells, Pig brain tissue, Mouse brain tissue, rat cerebellum tissue, mouse cerebellum tissue
Positive IHC detected inhuman liver cancer tissue, mouse brain tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66120-1-Ig targets Aldolase C in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Aldolase C fusion protein Ag6779
Full Name aldolase C, fructose-bisphosphate
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC003613
Gene Symbol ALDOC
Gene ID (NCBI) 230
RRIDAB_2881519
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involed in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).

Protocols

Product Specific Protocols
WB protocol for Aldolase C antibody 66120-1-IgDownload protocol
IHC protocol for Aldolase C antibody 66120-1-IgDownload protocol
IF protocol for Aldolase C antibody 66120-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols