Aldolase C Monoclonal antibody

Aldolase C Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

5H7A5

Cat no : 66120-1-Ig

Synonyms

ALDOC, Aldolase C, Brain type aldolase, Fructose bisphosphate aldolase C



Tested Applications

Positive WB detected inhuman heart tissue, human brain tissue
Positive IHC detected inhuman liver cancer tissue, mouse brain tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66120-1-Ig is Aldolase C Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Aldolase C fusion protein Ag6779
Full Name aldolase C, fructose-bisphosphate
Calculated molecular weight 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC003613
Gene symbol ALDOC
Gene ID (NCBI) 230
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involed in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).

Protocols

Product Specific Protocols
WB protocol for Aldolase C antibody 66120-1-IgDownload protocol
IHC protocol for Aldolase C antibody 66120-1-IgDownload protocol
IF protocol for Aldolase C antibody 66120-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols