Angiopoietin 1 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• Angiopoietin 1 Polyclonal antibody (27093-1-AP)

• 

  WB analysis using 27093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27093-1-AP (Angiopoietin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27093-1-AP (Angiopoietin 1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 27093-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27093-1-AP (Angiopoietin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27093-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27093-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 27093-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Angiopoietin 1 antibody (27093-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Angiopoietin 1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 27093-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ANGPT1, AGP1, AGPT, ANG 1, ANG1

---

Tested Applications

• Positive WB detected in: HUVEC cells, HeLa cells, U-87 MG cells, mouse heart tissue, rat heart tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

27093-1-AP targets Angiopoietin 1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25786

  Product name: Recombinant human ANGPT1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 16-67 aa of BC152411

  Sequence: HIGCSNQRRSPENSGRRYNRIQHGQCAYTFILPEHDGNCRESTTDQYNTNAL

• Full Name: angiopoietin 1
• Calculated Molecular Weight: 498 aa, 58 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC152411
• Gene Symbol: Angiopoietin 1
• Gene ID (NCBI): 284
• RRID: AB_3085925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15389
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Dose Response; Simultaneous Determination of Six Components in Zhuqi Piantan Granules and the Protective Effect of Combined Electroacupuncture on Cerebral Injury in Rats With Ischemic Stroke.; Authors - Ping Yang
rat	WB	Basic Res Cardiol; Investigating the cause of cardiovascular dysfunction in chronic kidney disease: capillary rarefaction and inflammation may contribute to detrimental cardiovascular outcomes; Authors - Siavash Beikoghli Kalkhoran
rat	WB	Brain Res Bull; Beneficial effects of electro-acupuncture treatment in photothrombotic ischemia model rats by remodeling neurovascular unit.; Authors - Zuanfang Li
mouse, rat	WB,IHC	J Nanobiotechnology; Dual-function MgSrCeAl-LDH nanosheets enable both ROS clearance and enhanced angiogenesis for myocardial ischemia/reperfusion injury therapy.; Authors - Jiayi Shen
human	WB,IF	Adv Healthc Mater; Targeted Anti-Inflammatory Nanozymes with Pro-Angiogenic Activity for Myocardial Infarction Therapy; Authors - Wenling Yang
		Phytomedicine; Fuhu Lijie Tang treats rheumatoid arthritis through multitarget therapy from autoantigen formation to bone destruction; Authors - Zhongliu Yao