Autophagy Essentials Antibody Kit




Target dependent


Target dependent



Cat no : PK30004

Product Information

The Autophagy Essentials Antibody Kit provides a cost-effective tool for studying key proteins involved in the autophagy pathway. Perfect for researchers starting a new project, screening multiple prospective targets or those who simply require less volume.

Kit Components

The Autophagy Essentials Antibody Kit contains antibodies against 5 key protein targets playing critical roles in the autophagy pathway. 

AntigenCatalog No.Host, clonalityTested ReactivityApplicationsVolume
Beclin 166665-1-Ig            Mouse monoclonalH, M, RWB, IHC, IF, ELISA20 uL
LC381004-1-RR            Rabbit monoclonalH, M, R, PgWB, IHC, IF, ELISA20 uL
p6280294-1-RR            Rabbit monoclonalHWB, IP, IHC, IF, ELISA20 uL
ATG581803-1-RR            Rabbit monoclonalH, M, RWB, IP, IHC, ELISA20 uL
LAMP121997-1-AP            Rabbit polyclonalHWB, IP, IHC, ELISA20 uL

Also see our 'Autophagy Expanded Antibody Kit' on the following page


Store at -20°C. Stable for one year from the date of receipt.

Background Information

Autophagy is a highly dynamic process consisting of the following three steps: (1) autophagosome formation, (2) autophagosome-lysosome fusion, and (3) degradation. It can be induced by multiple signaling pathways related to various triggers including nutrient deprivation, growth factor signaling, and cellular stress. The process of autophagosome formation proceeds through the steps of initiation, nucleation, elongation, closure, and ultimately fusion, each of which is regulated by various ATG proteins. 

The ideal approach for measuring autophagy is to assess autophagic flux, which represents the rate of degradation of the autophagic pathway. The most widely used method for measuring autophagic flux is to detect the processing of the autophagosomal membrane protein, LC3. Analyzing autophagy substrates such as p62/SQSTM1 is often recommended in addition to measuring LC3-II turnover for accurate assessment of autophagic flux. The fusion of autophagosomes with lysosomes can be monitored by analyzing the autophagosomal marker LC3 and the lysosomal marker, LAMP simultaneously. 

Standard Protocols

Click here to view our standard protocols for various applications including WB, IP, IHC, IF, FC, and ELISA.

Cited in Article as

PK30004, Autophagy Essentials Antibody Kit, Proteintech, IL, USA