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- KD/KO Validated
LC3 Recombinant antibody
LC3 Recombinant Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat, Pig
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
5P12
Cat no : 81004-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, Chloroquine treated HEK-293 cells, Chloroquine treated HeLa cells, pig brain tissue, rat brain tissue, mouse brain tissue |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Chloroquine treated HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF) | IF : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Product Information
81004-1-RR targets LC3 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
Tested Reactivity | Human, Mouse, Rat, Pig |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | LC3 fusion protein Ag6144 |
Full Name | microtubule-associated protein 1 light chain 3 beta |
Calculated molecular weight | 15 kDa |
Observed molecular weight | 14-18 kDa |
GenBank accession number | BC067797 |
Gene symbol | MAP1LC3B |
Gene ID (NCBI) | 81631 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane.
Protocols
Product Specific Protocols | |
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WB protocol for LC3 antibody 81004-1-RR | Download protocol |
IHC protocol for LC3 antibody 81004-1-RR | Download protocol |
IF protocol for LC3 antibody 81004-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |