|Positive WB detected in||U-937 cells|
|Positive IHC detected in||human gliomas tissue, human pancreas tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21997-1-AP is LAMP1 Fusion Protein expressed in E. coli.
|Cited Reactivity||human, turtle|
|Host / Isotype||Rabbit / IgG|
|Immunogen||LAMP1 fusion protein Ag17367|
|Full Name||lysosomal-associated membrane protein 1|
|Calculated molecular weight||417 aa, 45 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC021288|
|Gene ID (NCBI)||3916|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.
Fish Shellfish Immunol
Identification and functional characterization of interferon-γ-inducible lysosomal thiol reductase (GILT) gene in common Chinese cuttlefish Sepiella japonica.
Novel half-sandwich iridium OˆC (carbene)-Complexes: In vitro and in vivo tumor growth suppression and pro-apoptosis via ROS-mediated cross-talk between mitochondria and lysosomes.
Dysregulated autophagy as a new aspect of the molecular pathogenesis of Krabbe disease.
Oxid Med Cell Longev
Enhanced p62-NRF2 Feedback Loop due to Impaired Autophagic Flux Contributes to Arsenic-Induced Malignant Transformation of Human Keratinocytes.
Aging (Albany NY)
Cytoplasmic PCNA is located in the actin belt and involved in osteoclast differentiation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Azita (Verified Customer) (06-17-2021)
Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by LAMP1 Polyclonal antibody at dilution of 1:50 showed strong labelling.
Mayru (Verified Customer) (04-30-2019)
The antibody works great for detection of LAMP1 protein expression using Western blot analysis.