Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells, L02 cells, MCF-7 cells, MDA-MB-231 cells, U-937 cells |
| Positive IHC detected in | human liver cancer tissue, human colon cancer tissue, human gliomas tissue, human pancreas tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human ovary tumor tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21997-1-AP targets CD107a / LAMP1 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig, monkey, zebrafish, bovine, turtle |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17367 Product name: Recombinant human CD107a protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 35-377 aa of BC021288 Sequence: NGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLL Predict reactive species |
| Full Name | lysosomal-associated membrane protein 1 |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 90-120 kDa |
| GenBank Accession Number | BC021288 |
| Gene Symbol | LAMP1 |
| Gene ID (NCBI) | 3916 |
| ENSEMBL Gene ID | ENSG00000185896 |
| RRID | AB_2878966 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11279 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD107a / LAMP1 antibody 21997-1-AP | Download protocol |
| IHC protocol for CD107a / LAMP1 antibody 21997-1-AP | Download protocol |
| WB protocol for CD107a / LAMP1 antibody 21997-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD107a/LAMP1 polyclonal antibody (Cat# 21997-1-AP) has accumulated 223 citations. Publications appear in high-impact journals including Autophagy, Nature Communications, Advanced Science, Cell Reports, EMBO Molecular Medicine, PNAS, Oncogene, Cell Death & Disease, Redox Biology, Molecular Therapy, Free Radical Biology and Medicine, and Theranostics. Research fields encompass autophagy and lysosomal biology, cancer, neurodegeneration (Alzheimer's, Parkinson's), ferroptosis, lipid metabolism, inflammation, viral infections, and cardiovascular disease.
| Species | Application | Title |
|---|---|---|
Cell Metab Oral GLP-1 receptor agonist promotes astrocyte-neuron lactate and lipid transfer with neuroprotective effects. | ||
Immunity The Endotoxin Delivery Protein HMGB1 Mediates Caspase-11-Dependent Lethality in Sepsis. | ||
Bone Res Chaperone-mediated autophagy directs a dual mechanism to balance premature senescence and senolysis to prevent intervertebral disc degeneration | ||
Autophagy Lipotoxic hepatocyte-derived UBQLN1-enriched small extracellular vesicles activate hepatic stellate cells to promote hepatic fibrosis. | ||
Autophagy Targeting the SNAI1-LAMP3 axis to restore lysosomal function and alleviate autophagic flux impairment to delay retinal degeneration. |
Reviews
What customers say
All four reviews are 5-star. Users report excellent performance for Western blot and immunohistochemistry. WB results show clear LAMP1 bands in human breast cancer cells (1:1000) and H4 cells (1:1000). IHC on fixed NSC-34 cells at 1:50 yielded strong labeling. One user noted it "works great" across N2A, 293T cells, and tissue. Overall, reviewers consistently highlight reliable detection and strong signal across cell lines and tissues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Zakia (Verified Customer) (05-22-2024) | Works great.
|
Marina (Verified Customer) (08-08-2022) | Western blot against LAMP1 in human breast cancer cell line HCC1937. 1:1000 dilution, overnight incubation at 4ºC. A clear band can be observed between 95 and 130 kDa. Ponceau S staining is included as total protein loading control.
 |
Azita (Verified Customer) (06-17-2021) | Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by LAMP1 Polyclonal antibody at dilution of 1:50 showed strong labelling.
|
Mayru (Verified Customer) (04-30-2019) | The antibody works great for detection of LAMP1 protein expression using Western blot analysis.
|
