CD107a / LAMP1 Polyclonal antibody
CD107a / LAMP1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human and More (4)
WB, IP, IHC, FC, ELISA
Cat no : 21997-1-AP
Validation Data Gallery
|Positive WB detected in||HepG2 cells, U937 cells|
|Positive IHC detected in||human liver cancer tissue, human pancreas tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|WB||See 55 publications below|
|IHC||See 4 publications below|
|IF||See 29 publications below|
|IP||See 1 publications below|
|FC||See 1 publications below|
21997-1-AP targets CD107a / LAMP1 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, bovine, monkey, turtle, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD107a / LAMP1 fusion protein Ag17367|
|Full Name||lysosomal-associated membrane protein 1|
|Calculated molecular weight||417 aa, 45 kDa|
|Observed molecular weight||90-120 kDa|
|GenBank accession number||BC021288|
|Gene ID (NCBI)||3916|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.
|Product Specific Protocols|
|WB protocol for CD107a / LAMP1 antibody 21997-1-AP||Download protocol|
|IHC protocol for CD107a / LAMP1 antibody 21997-1-AP||Download protocol|
|Click here to view our Standard Protocols|
The Endotoxin Delivery Protein HMGB1 Mediates Caspase-11-Dependent Lethality in Sepsis.
LAMP3 induces lysosome-dependent cell death by impairing autophagic caspase-8 degradation in Sjögren's salivary glands
Mismatch repair deficiency predicts response to HER2 blockade in HER2-negative breast cancer.
Sci Total Environ
TFEB coordinates autophagy and pyroptosis as hepatotoxicity responses to ZnO nanoparticles
PNPLA2 mobilizes retinyl esters from retinosomes and promotes the generation of 11-cis-retinal in the visual cycle
LAMP3 inhibits autophagy and contributes to cell death by lysosomal membrane permeabilization.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Marina (Verified Customer) (08-08-2022)
Western blot against LAMP1 in human breast cancer cell line HCC1937. 1:1000 dilution, overnight incubation at 4ºC. A clear band can be observed between 95 and 130 kDa. Ponceau S staining is included as total protein loading control.
Azita (Verified Customer) (06-17-2021)
Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by LAMP1 Polyclonal antibody at dilution of 1:50 showed strong labelling.
Mayru (Verified Customer) (04-30-2019)
The antibody works great for detection of LAMP1 protein expression using Western blot analysis.