CD80 Monoclonal antibody

CD80 Monoclonal Antibody for WB, ELISA
Cat No. 66406-1-Ig
Clone No.1E2F10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig and More (2)

Applications

WB, IF, ELISA

1E2F10, Activation B7 1 antigen, Activation B7-1 antigen, B7, B7 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inRaji cells, DC2.4 cells, Daudi cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66406-1-Ig targets CD80 in WB, IF, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivityhuman, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag5615

Product name: Recombinant human B7-1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 36-228 aa of BC042665

Sequence: IHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQT

Predict reactive species
Full Name CD80 molecule
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC042665
Gene Symbol CD80
Gene ID (NCBI) 941
RRIDAB_2827408
Conjugate Unconjugated
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP33681
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Protocols

Product Specific Protocols
WB protocol for CD80 antibody 66406-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Acta Pharm Sin B

Converting bacteria into autologous tumor vaccine via surface biomineralization of calcium carbonate for enhanced immunotherapy

Authors - Lina Guo
humanIF

Cell Rep Med

Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers

Authors - Bingxin Liu
mouseWB

ACS Appl Mater Interfaces

Surface Coating of Pulmonary siRNA Delivery Vectors Enabling Mucus Penetration, Cell Targeting, and Intracellular Radical Scavenging for Enhanced Acute Lung Injury Therapy.

Authors - Jiaojiao Zhu

J Nanobiotechnology

Dual-modality immune nano-activator harnessing Mn2⁺ and quercetin to potentiate the cGAS-STING pathway for advanced cancer metalloimmunotherapy

Authors - Shanshan Ma
mouse,human

Theranostics

ILT4 inhibition prevents TAM- and dysfunctional T cell-mediated immunosuppression and enhances the efficacy of anti-PD-L1 therapy in NSCLC with EGFR activation.

Authors - Xiaozheng Chen
humanWB

Int J Biol Macromol

Study on the mechanism of macrophages activated by phosphoesterified rehmanniae polysaccharide on human gastric cancer cells

Authors - Xianglong Liu
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