CD80 Monoclonal antibody

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• CD80 Monoclonal antibody (66406-1-Ig)

CD80 Monoclonal Antibody for WB, ELISA

Cat No. 66406-1-Ig Copied!

Clone No. 1E2F10

Publications (125)

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Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig and More (2)

Applications

WB, IF, ELISA

Synonyms

1E2F10, Activation B7 1 antigen, Activation B7-1 antigen, B7, B7 1

• 

  WB analysis of DC2.4 using 66406-1-Ig

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80/B7-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Raji using 66406-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80 / B7-1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Daudi using 66406-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80 / B7-1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Raji cells, DC2.4 cells, Daudi cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66406-1-Ig targets CD80 in WB, IF, ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Cited Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5615

  Product name: Recombinant human B7-1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 36-228 aa of BC042665

  Sequence: IHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQT

• Full Name: CD80 molecule
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC042665
• Gene Symbol: CD80
• Gene ID (NCBI): 941
• RRID: AB_2827408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P33681
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Publications

What published studies show

The CD80 Monoclonal antibody (1E2F10), catalog number 66406-1-Ig, has 125 citations. These appear in journals including ACS Nano, Biomaterials, Nature Communications, Theranostics, Advanced Science, Cell Reports, Journal of Immunotherapy of Cancer, Acta Pharmaceutica Sinica B, and Frontiers in Immunology. Research fields center on macrophage polarization (M1/M2), tumor-associated macrophages, and cancer immunotherapy, with additional work in inflammatory diseases (colitis, arthritis, atherosclerosis), immune checkpoint regulation, and nanomedicine-based drug delivery. Applications cited include WB, IF, and ELISA.

Automatically generated from the 125 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Adv Mater; An OMV-Based Nanovaccine as Antigen Presentation Signal Enhancer for Cancer Immunotherapy; Authors - Yichao Lu
		J Extracell Vesicles; Dendritic Cell Derived-Extracellular Vesicles Engineered to Express Interleukin-12 and Anti-CTLA-4 on Their Surface for Combinational Cancer Immunotherapy; Authors - Jiangbin Chen
mouse	WB	Nat Commun; Neuropeptide SP protects against colitis and linked anxiety-like behavior through the putative roles of gut microbiota and metabolite inositol.; Authors - Jing Lan
human	IF	ACS Nano; Biomimetic Nanoparticles Simultaneously Targeting Modulation of Lipid Metabolism and Phenotype of Macrophages for Programmed Atherosclerosis Management; Authors - Xiaoyan Dong
rat	IF	ACS Nano; Engineered Magneto-Piezoelectric Nanoparticles-Enhanced Scaffolds Disrupt Biofilms and Activate Oxidative Phosphorylation in Icam1+ Macrophages for Infectious Bone Defect Regeneration; Authors - Hao Wu
mouse	WB	ACS Nano; Virus-Mimetic Extracellular-Vesicle Vaccine Boosts Systemic and Mucosal Immunity via Immune Recruitment; Authors - Jingru Li