BACH2 Polyclonal antibody

• Featured Product
• KD/KO Validated

BACH2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 27635-1-AP

Synonyms

BACH2, BTB and CNC homolog 2

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Validation Data Gallery

• 

  WB analysis of Daudi using 27635-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 27635-1-AP (BACH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 27635-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 27635-1-AP (BACH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 27635-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 27635-1-AP (BACH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Daudi cells, Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

27635-1-AP targets BACH2 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BACH2 fusion protein Ag26526
• Full Name: BTB and CNC homology 1, basic leucine zipper transcription factor 2
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: NM_001170794
• Gene Symbol: BACH2
• Gene ID (NCBI): 60468
• RRID: AB_2880934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BACH2, also named as Transcription regulator protein BACH2, is a 841 amino acid protein, which is widely expressed within the B-lymphoid lineage, except in plasma cells. BACH2 contains an N-terminal BTB domain involved in its transcriptional function, but instead of having zinc fingers at the C-terminus, it binds to DNA through a basic leucine zipper motif. BACH2 binds DNA consensus sequences, termed MARE motifs, by forming a heterodimer with small leucine zipper MAF proteins such as MAFK, MAFG, and MAFF. The calculated molecular weight of BACH2 is 92 kDa, but the post-modifiction of BACH2 is about 120-130 kDa. (PMID: 24277074 )

Publications

Species	Application	Title
human	WB	FEBS Open Bio; Bach2 overexpression represses Th9 cell differentiation by suppressing IRF4 expression in systemic lupus erythematosus.; Authors - Yujun Sheng ; KD Validated
human	WB	Cell Rep; Class I HDAC inhibitors enhance antitumor efficacy and persistence of CAR-T cells by activation of the Wnt pathway; Authors - Meng Zhu