BAG6 Polyclonal antibody

• Featured Product
• KD/KO Validated

BAG6 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 26417-1-AP

Synonyms

BAG 6, BAG6, BAT3, D6S52E, G3, HLA B associated transcript 3, Protein G3

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Validation Data Gallery

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  WB analysis using 26417-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26417-1-AP (BAG6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 26417-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26417-1-AP (BAG6 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 26417-1-AP

  IP result of anti-BAG6 (IP:26417-1-AP, 4ug; Detection:66661-1-Ig 1:600) with A431 cells lysate 1920 ug.

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  IHC staining of human breast cancer using 26417-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26417-1-AP (BAG6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26417-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26417-1-AP (BAG6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 26417-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26417-1-AP (BAG6 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 26417-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26417-1-AP (BAG6 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 26417-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BAG6 antibody (26417-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, HeLa cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human breast cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 26417-1-AP is BAG6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BAG6 fusion protein Ag24525
• Full Name: HLA-B associated transcript 3
• Calculated Molecular Weight: 119 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC003133
• Gene Symbol: BAT3
• Gene ID (NCBI): 7917
• RRID: AB_2880508
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAT3 also known as Scythe or BAG6, is a nuclear protein implicated in the control of apoptosis and natural killer (NK) cell-dendritic cell (DC) interaction. BAT3 was first discovered as a member of a group of genes located within the class III region of the human major histocompatibility complex on chromosome 6, and has been extensively studied for its role in regulating apoptosis under various stress conditions such as DNA damage and endoplasmic reticulum-related stress. BAT3 has been shown to be required for p53 acetylation, which is critical for the enhancement of p53 transcriptional activity in response to DNA damage. In addition, BAT3 is involved in the regulation of development and reproduction of mammals by acting as a co-chaperone of the heat shock protein HSP70.

Publications

Species	Application	Title
	IHC	Clin Transl Med; Deubiquitinase USP19 modulates apoptotic calcium release and endoplasmic reticulum stress by deubiquitinating BAG6 in triple negative breast cancer; Authors - Xiaoqiang Zhang
human	IF	FASEB J; BAG6 promotes PINK1 signaling pathway and is essential for mitophagy.; Authors - Romain Ragimbeau
human	WB,IP,CoIP,IF	Mol Cell Biol; The VCP-UBXN1 complex mediates triage of ubiquitylated cytosolic proteins bound to the BAG6 complex.; Authors - Rakesh Ganji ; KD Validated
human	WB,IF	Exp Eye Res; Retinitis pigmentosa 2 pathogenic mutants degrade through BAG6/HUWE1 complex.; Authors - Jing Zhang ; KD Validated
human	WB,IF	Front Immunol; BAG6 negatively regulates the RLR signaling pathway by targeting VISA/MAVS; Authors - Jing-Ping Huang ; KO Validated
human	WB	EMBO Rep; C9orf72 protein quality control by UBR5-mediated heterotypic ubiquitin chains; Authors - Julia Jülg