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  • KD/KO Validated

BAG6 Polyclonal antibody

BAG6 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 26417-1-AP

Synonyms

BAG 6, BAG6, BAT3, D6S52E, G3, HLA B associated transcript 3, Protein G3



Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, HeLa cells
Positive IP detected inA431 cells
Positive IHC detected inhuman breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26417-1-AP is BAG6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAG6 fusion protein Ag24525
Full Name HLA-B associated transcript 3
Calculated molecular weight 119 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC003133
Gene symbol BAT3
Gene ID (NCBI) 7917
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BAT3 also known as Scythe or BAG6, is a nuclear protein implicated in the control of apoptosis and natural killer (NK) cell-dendritic cell (DC) interaction. BAT3 was first discovered as a member of a group of genes located within the class III region of the human major histocompatibility complex on chromosome 6, and has been extensively studied for its role in regulating apoptosis under various stress conditions such as DNA damage and endoplasmic reticulum-related stress. BAT3 has been shown to be required for p53 acetylation, which is critical for the enhancement of p53 transcriptional activity in response to DNA damage. In addition, BAT3 is involved in the regulation of development and reproduction of mammals by acting as a co-chaperone of the heat shock protein HSP70.

Protocols

Product Specific Protocols
WB protocol for BAG6 antibody 26417-1-APDownload protocol
IHC protocol for BAG6 antibody 26417-1-APDownload protocol
IF protocol for BAG6 antibody 26417-1-APDownload protocol
IP protocol for BAG6 antibody 26417-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP,CoIP,IF

Mol Cell Biol

The VCP-UBXN1 complex mediates triage of ubiquitylated cytosolic proteins bound to the BAG6 complex.

Authors - Rakesh Ganji
  • KD Validated
humanIF

FASEB J

BAG6 promotes PINK1 signaling pathway and is essential for mitophagy.

Authors - Romain Ragimbeau