|Positive WB detected in||A431 cells, HEK-293 cells, HeLa cells|
|Positive IP detected in||A431 cells|
|Positive IHC detected in||human breast cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 26417-1-AP is BAG6 Fusion Protein expressed in E. coli.
|Host / Isotype||Rabbit / IgG|
|Immunogen||BAG6 fusion protein Ag24525|
|Full Name||HLA-B associated transcript 3|
|Calculated molecular weight||119 kDa|
|Observed molecular weight||150 kDa|
|GenBank accession number||BC003133|
|Gene ID (NCBI)||7917|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
BAT3 also known as Scythe or BAG6, is a nuclear protein implicated in the control of apoptosis and natural killer (NK) cell-dendritic cell (DC) interaction. BAT3 was first discovered as a member of a group of genes located within the class III region of the human major histocompatibility complex on chromosome 6, and has been extensively studied for its role in regulating apoptosis under various stress conditions such as DNA damage and endoplasmic reticulum-related stress. BAT3 has been shown to be required for p53 acetylation, which is critical for the enhancement of p53 transcriptional activity in response to DNA damage. In addition, BAT3 is involved in the regulation of development and reproduction of mammals by acting as a co-chaperone of the heat shock protein HSP70.
|Product Specific Protocols|
|WB protocol for BAG6 antibody 26417-1-AP||Download protocol|
|IHC protocol for BAG6 antibody 26417-1-AP||Download protocol|
|IF protocol for BAG6 antibody 26417-1-AP||Download protocol|
|IP protocol for BAG6 antibody 26417-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mol Cell Biol
The VCP-UBXN1 complex mediates triage of ubiquitylated cytosolic proteins bound to the BAG6 complex.
BAG6 promotes PINK1 signaling pathway and is essential for mitophagy.