• Featured Product
  • KD/KO Validated

BAG6 Polyclonal antibody

BAG6 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)





Cat no : 26417-1-AP


BAG 6, BAG6, BAT3, D6S52E, G3, HLA B associated transcript 3, Protein G3

Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, HeLa cells
Positive IP detected inA431 cells
Positive IHC detected inhuman breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26417-1-AP is BAG6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAG6 fusion protein Ag24525
Full Name HLA-B associated transcript 3
Calculated Molecular Weight 119 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC003133
Gene Symbol BAT3
Gene ID (NCBI) 7917
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAT3 also known as Scythe or BAG6, is a nuclear protein implicated in the control of apoptosis and natural killer (NK) cell-dendritic cell (DC) interaction. BAT3 was first discovered as a member of a group of genes located within the class III region of the human major histocompatibility complex on chromosome 6, and has been extensively studied for its role in regulating apoptosis under various stress conditions such as DNA damage and endoplasmic reticulum-related stress. BAT3 has been shown to be required for p53 acetylation, which is critical for the enhancement of p53 transcriptional activity in response to DNA damage. In addition, BAT3 is involved in the regulation of development and reproduction of mammals by acting as a co-chaperone of the heat shock protein HSP70.


Product Specific Protocols
WB protocol for BAG6 antibody 26417-1-APDownload protocol
IHC protocol for BAG6 antibody 26417-1-APDownload protocol
IF protocol for BAG6 antibody 26417-1-APDownload protocol
IP protocol for BAG6 antibody 26417-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Clin Transl Med

Deubiquitinase USP19 modulates apoptotic calcium release and endoplasmic reticulum stress by deubiquitinating BAG6 in triple negative breast cancer

Authors - Xiaoqiang Zhang


BAG6 promotes PINK1 signaling pathway and is essential for mitophagy.

Authors - Romain Ragimbeau

Mol Cell Biol

The VCP-UBXN1 complex mediates triage of ubiquitylated cytosolic proteins bound to the BAG6 complex.

Authors - Rakesh Ganji
  • KD Validated

Exp Eye Res

Retinitis pigmentosa 2 pathogenic mutants degrade through BAG6/HUWE1 complex.

Authors - Jing Zhang
  • KD Validated

Front Immunol

BAG6 negatively regulates the RLR signaling pathway by targeting VISA/MAVS

Authors - Jing-Ping Huang
  • KO Validated


C9orf72 protein quality control by UBR5-mediated heterotypic ubiquitin chains

Authors - Julia Jülg