The protein was expressed as 6xHis-tagged fusion protein by E.Coli and purified by Ni-sepharose. The purified protein was resolved in PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4 ) added with 15% glycerol. The elution buffer contain 300mM imidazole.
Shipping and Storage
Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
Reconstitution with 200 μl 50% glycerol solution is recommended for longer term
storage (see Stability and Storage for more details).
If a different concentration is needed for your purposes please adjust the reconstitution
volume as required (please note: the ion concentration of the final solution will
vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash
down the sides of the vial to ensure full recovery of the protein into solution.
The product is shipped with ice packs. Upon receipt, store it immediately at -20°C to -80°C.
Stability and Storage
Aliquot and store at -20°C to -80°C for up to 6 months. Avoid freeze thaw cycles.
Storage of Reconstituted Protein
Short-term storage: Store at 2-8°C for (1-2 weeks).
Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer
containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw