BAK Polyclonal antibody

BAK Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


Human, mouse, rat and More (1)





Cat no : 29552-1-AP


Apoptosis regulator BAK, BAK, BAK LIKE, BAK1, CDN1

Tested Applications

Positive WB detected inA431 cells, Neuro-2a cells, HEK-293 cells, HepG2 cells, Jurkat cells, MOLT-4 cells, 4T1 cells, ROS1728 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

29552-1-AP targets BAK in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAK fusion protein Ag31042
Full Name BCL2-antagonist/killer 1
Calculated molecular weight 23 kDa
Observed molecular weight23 kDa
GenBank accession numberNM_001188
Gene symbol BAK1
Gene ID (NCBI) 578
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.


Product Specific Protocols
WB protocol for BAK antibody 29552-1-APDownload protocol
IHC protocol for BAK antibody 29552-1-APDownload protocol
IF protocol for BAK antibody 29552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Chem Biol Interact

Hsa_circ_0005050 interacts with ILF3 and affects cell apoptosis and proliferation by disrupting the balance between p53 and p65

Authors - Jingwen Tan

Med Oncol

PU.1 negatively regulates tumorigenesis in non-small-cell lung cancer

Authors - Xiaoxiao Liu

Exp Hematol

Platycodin D induces proliferation inhibition and mitochondrial apoptosis in diffuse large B-cell lymphoma

Authors - Pu Liu