BANP Polyclonal antibody

BANP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16393-1-AP

Synonyms

BANP, BEND1, Protein BANP, SMAR1, SMARBP1

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Validation Data Gallery

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  WB analysis using 16393-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16393-1-AP (BANP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 16393-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16393-1-AP (BANP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16393-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16393-1-AP (BANP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, K-562 cells, MCF-7 cells, U2OS cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

16393-1-AP targets BANP in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BANP fusion protein Ag9484
• Full Name: BTG3 associated nuclear protein
• Calculated Molecular Weight: 545 aa, 59 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC009424
• Gene Symbol: BANP
• Gene ID (NCBI): 54971
• RRID: AB_2061101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Hum Mol Genet; TBN improves motor function and prolongs survival in a TDP-43M337V mouse model of ALS.; Authors - Chunhui Huang
human	WB,IHC	Oncotarget; The suppressing effects of BTG3 expression on aggressive behaviors and phenotypes of colorectal cancer: An in vitro and vivo study.; Authors - Hua-Chuan Zheng