BAP29 Polyclonal antibody

BAP29 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 15796-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

BCR-associated protein 29, B-cell receptor-associated protein 29, BCAP29

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inJurkat cells, COLO 320 cells, Raji cells, U-87 MG cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15796-1-AP targets BAP29 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAP29 fusion protein Ag8505 Predict reactive species
Full Name B-cell receptor-associated protein 29
Calculated Molecular Weight 241 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC008478
Gene Symbol BAP29
Gene ID (NCBI) 55973
RRIDAB_2065118
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHQ4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BAP29 antibody 15796-1-APDownload protocol
IHC protocol for BAP29 antibody 15796-1-APDownload protocol
IF protocol for BAP29 antibody 15796-1-APDownload protocol
FC protocol for BAP29 antibody 15796-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Legionella uses host Rab GTPases and BAP31 to create a unique ER niche

Authors - Attinder Chadha

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Xin (Verified Customer) (03-19-2021)

The protein ran slightly lower than the predicted size (28kDa), it was detected somewhere around 24kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: A549 cells
BAP29 Antibody Western Blot validation (1:1000 dilution) in A549 cells (Cat no:15796-1-AP)
Loading...