BDNF Polyclonal antibody

BDNF Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

69

Reviews (1)

Cat no : 28205-1-AP

Synonyms

Abrineurin, BDNF

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Validation Data Gallery

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  WB analysis using 28205-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28205-1-AP (BDNF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 28205-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 28205-1-AP (BDNF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 28205-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 28205-1-AP (BDNF antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 28205-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 28205-1-AP (BDNF antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 28205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28205-1-AP (BDNF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 28205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28205-1-AP (BDNF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28205-1-AP (BDNF antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28205-1-AP (BDNF antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-12 using 28205-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using 28205-1-AP (BDNF antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: C6 cells, SH-SY5Y cells, PC-12 cells
• Positive IHC detected in: human gliomas tissue, mouse brain tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 60 publications below
• IHC: See 3 publications below
• IF: See 8 publications below

Product Information

28205-1-AP targets BDNF in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BDNF fusion protein Ag28276
• Full Name: brain-derived neurotrophic factor
• Calculated molecular weight: 247 aa, 28 kDa
• Observed molecular weight: 28-30 kDa
• GenBank accession number: BC029795
• Gene symbol: BDNF
• Gene ID (NCBI): 627
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).

Publications

Species	Application	Title
		Autophagy; Trehalose induces autophagy via lysosomal-mediated TFEB activation in models of motoneuron degeneration.; Authors - Paola Rusmini
rat	WB	Neurobiol Stress; Low-dose S-ketamine exerts antidepressant-like effects via enhanced hippocampal synaptic plasticity in postpartum depression rats.; Authors - Zhuoyu Ren
mouse	WB	Brain Behav Immun; IL-33 in the basolateral amygdala integrates neuroinflammation into anxiogenic circuits via modulating BDNF expression.; Authors - Xiao Zhuang
mouse	WB	J Neuroinflammation; Inhibition of P2X4R attenuates white matter injury in mice after intracerebral hemorrhage by regulating microglial phenotypes.; Authors - Xiongjie Fu
rat	IHC	FASEB J; The protective function of taurine on pesticide-induced permanent neurodevelopmental toxicity in juvenile rats.; Authors - Fukai Liu
mouse	WB	Biochem Pharmacol; Developmental nicotine exposure elicits multigenerational disequilibria in proBDNF proteolysis and glucocorticoid signaling in the frontal cortices, striata, and hippocampi of adolescent mice.; Authors - Jordan M Buck

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tanusree (Verified Customer) (12-18-2019)

Worked well in WB at 1:500 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain