Filter: AllWBIHCIF-P

WB analysis using 14647-1-AP (same clone as 14647-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 14647-1-AP (same clone as 14647-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.
WB analysis of mouse skeletal muscle using 14647-1-AP (same clone as 14647-1-PBS)
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14647-1-AP (BIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 14647-1-AP (same clone as 14647-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 14647-1-AP (same clone as 14647-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.
IHC staining of human osteosarcoma using 14647-1-AP (same clone as 14647-1-PBS)
Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14647-1-AP (BIN1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.
IHC staining of human osteosarcoma using 14647-1-AP (same clone as 14647-1-PBS)
Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14647-1-AP (BIN1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14647-1-AP (BIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BIN1 antibody (14647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 14647-1-AP (same clone as 14647-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BIN1 antibody (14647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14647-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

BIN1 Polyclonal antibody, PBS Only

BIN1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 14647-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

AMPH2, Amphiphysin II, Amphiphysin like protein, Amphiphysin-like protein, AMPHL

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

14647-1-PBS targets BIN1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6240 Product name: Recombinant human BIN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-439 aa of BC004101 Sequence: MAEMGSKGVTAGKIASNVQKKLTRAQEKVLQKLGKADETKDEQFEQCVQNFNKQLTEGTRLQKDLRTYLASVKAMHEASKKLNECLQEVYEPDWPGRDEANKIAENNDLLWMDYHQKLVDQALLTMDTYLGQFPDIKSRIAKRGRKLVDYDSARHHYESLQTAKKKDEAKIAKAEEELIKAQKVFEEMNVDLQEELPSLWNSRVGFYVNTFQSIAGLEENFHKEMSKLNQNLNDVLVGLEKQHGSNTFTVKAQPSDNAPAKGNKSPSPPDGSPAATPEIRVNHEPEPAGGATPGATLPKSPSQPAEASEVAGGTQPAAGAQEPGETAASEAASSSLPAVVVETFPATVNGTVEGGSGAGRLDLPPGFMFKVQAQHDYTATDTDELQLRAGDVVLVIPFQNPEEQDEGWLMGVKESDWNQHKELEKCRGVFPENFTERVP Predict reactive species
Full Name	bridging integrator 1
Calculated Molecular Weight	65 kDa
Observed Molecular Weight	50-65 kDa
GenBank Accession Number	BC004101
Gene Symbol	BIN1
Gene ID (NCBI)	274
RRID	AB_2243396
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O00499
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

BIN1 (Bridging integrator 1), also known as amphiphysin II or Myc box-dependent-interacting protein 1, is a ubiquitous nucleocytoplasmic adaptor protein that was identified initially as an MYC-interacting proapoptotic tumor suppressor. Alternative splicing of the gene results in multiple transcript variants encoding different isoforms. BIN1 is a key regulator of different cellular functions, including endocytosis and membrane recycling, cytoskeleton regulation, DNA repair, cell cycle progression, and apoptosis (PMID: 24590001).