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BIN2 Polyclonal antibody

BIN2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 14245-1-AP


BIN2, BRAP 1, BRAP1, bridging integrator 2

Tested Applications

Positive WB detected inJurkat cells, U-937 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman spleen tissue, mouse heart tissue, human heart tissue, human skin tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:2000-1:14000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14245-1-AP targets BIN2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BIN2 fusion protein Ag5502
Full Name bridging integrator 2
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 80-90 kDa
GenBank Accession NumberBC047686
Gene Symbol BIN2
Gene ID (NCBI) 51411
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAR proteins, which are characterized by a common N-terminal BAR domain, are a family of adaptor proteins implicated in a diverse set of cellular processes. BIN1 (bridging integrator 1) and BIN2 are BAR proteins. The human BIN2 gene is located at chromosome 4q22.1, and encodes a 565-amino acid protein with a predicted molecular weight of ~62 kDa. It shows preferential expression in hematopoietic tissues, with highest mRNA levels in spleen and peripheral blood leukocytes and also high levels in thymus, colon, and placenta. BIN2 has been shown to migrate with an apparent mobility of ~80 kDa, and interact with BIN1. (PMID: 10903846)


Product Specific Protocols
WB protocol for BIN2 antibody 14245-1-APDownload protocol
IHC protocol for BIN2 antibody 14245-1-APDownload protocol
IP protocol for BIN2 antibody 14245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Clin Invest

BIN2 orchestrates platelet calcium signaling in thrombosis and thrombo-inflammation.

Authors - Julia Volz
  • KO Validated