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BIN2 Polyclonal antibody

BIN2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 14245-1-AP

Synonyms

BIN2, BRAP 1, BRAP1, bridging integrator 2



Tested Applications

Positive WB detected inU-937 cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman spleen tissue, human heart tissue, human lung tissue, human ovary tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14245-1-AP targets BIN2 in WB, IP, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BIN2 fusion protein Ag5502
Full Name bridging integrator 2
Calculated molecular weight 62 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC047686
Gene symbol BIN2
Gene ID (NCBI) 51411
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BAR proteins, which are characterized by a common N-terminal BAR domain, are a family of adaptor proteins implicated in a diverse set of cellular processes. BIN1 (bridging integrator 1) and BIN2 are BAR proteins. The human BIN2 gene is located at chromosome 4q22.1, and encodes a 565-amino acid protein with a predicted molecular weight of ~62 kDa. It shows preferential expression in hematopoietic tissues, with highest mRNA levels in spleen and peripheral blood leukocytes and also high levels in thymus, colon, and placenta. BIN2 has been shown to migrate with an apparent mobility of ~80 kDa, and interact with BIN1. (PMID: 10903846)

Protocols

Product Specific Protocols
WB protocol for BIN2 antibody 14245-1-APDownload protocol
IHC protocol for BIN2 antibody 14245-1-APDownload protocol
IP protocol for BIN2 antibody 14245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

J Clin Invest

BIN2 orchestrates platelet calcium signaling in thrombosis and thrombo-inflammation.

Authors - Julia Volz
  • KO Validated