BLIMP1/PRDM1 Recombinant antibody, PBS Only

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  WB analysis using 84625-3-RR (same clone as 84625-3-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 84625-3-RR (BLIMP1/PRDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84625-3-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84625-3-RR (same clone as 84625-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using BLIMP1/PRDM1 antibody (84625-3-RR, Clone: 242030B1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84625-3-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 84625-3-RR (same clone as 84625-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using BLIMP1/PRDM1 antibody (84625-3-RR, Clone: 242030B1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84625-3-PBS in a different storage buffer formulation.

BLIMP1/PRDM1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 84625-3-PBS

Clone No.242030B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

BLIMP1, PRDM1, 242030B1, BLIMP 1, PRDI BF1

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Product Information

84625-3-PBS targets BLIMP1/PRDM1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BLIMP1/PRDM1 fusion protein Eg2625
• Full Name: PR domain containing 1, with ZNF domain
• Calculated Molecular Weight: 92 kDa
• Observed Molecular Weight: 96-110 kDa
• GenBank Accession Number: NM_001198.3
• Gene Symbol: PRDM1
• Gene ID (NCBI): 639
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O75626-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BLIMP1(B lymphocyte-induced maturation protein 1), also known as PRDM1(PR domain zinc finger protein 1), is an evolutionarily conserved transcriptional regulator originally described as a repressor of gene transcription(PMID: 35154079). BLIMP1/PRDM1 is widely expressed in multiple cell types including B lymphocytes, T lymphocytes, mammary luminal progenitor cells and skin epithelial cells. BLIMP1/PRDM1 is a master regulator of terminal B cell differentiation and may also function as a tumor suppressor in the pathogenesis of DLBCL, where it is frequently inactivated by mutations and deletions.