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Recombinant Human PRDM1 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

C-rFc

Activity

not tested

Cat no : Eg2625

Validation Data Gallery

  • Purity of Recombinant Human PRDM1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human PRDM1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human PRDM1 protein Asp45-Gln230 (Accession# O75626-1) with a rabbit IgG Fc tag at the C-terminus
GeneID 639
Accession O75626-1
PredictedSize 47.7 kDa
SDS-PAGE 48-52 kDa, reducing (R) condition
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

PRDM1 belongs to the PR/SET structural domain protein family and is a DNA-binding transcriptional repressor. PRDM1 has histone deacetylase binding activity and specifically binds promoter chromatin. PRDM1 acts as a deterrent to beta-interferon gene expression and binds specifically to the PRDI element in the promoter of the beta-interferon gene.

References:

1. Matthew A Smith, et al. (2010) J Immunol. 185(10):6058-67.
2. A D Keller, et al. (1991) Genes Dev. 5(5):868-79.