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BMP2 Antibody 17 Publications

Rabbit Polyclonal| Catalog number: 18933-1-AP

Featured Product KD/KO validated

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Con: 24 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

BMP2 Antibody WB HT-1080 cells 18933-1-AP

HT-1080 cells were subjected to SDS PAGE followed by western blot with 18933-1-AP (BMP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

BMP2 Antibody IHC human lung tissue 18933-1-AP

Immunohistochemical of paraffin-embedded human lung using 18933-1-AP(BMP2 antibody) at dilution of 1:100 (under 10x lens)

BMP2 Antibody IHC human lung tissue 18933-1-AP

Immunohistochemical of paraffin-embedded human lung using 18933-1-AP(BMP2 antibody) at dilution of 1:100 (under 40x lens)

BMP2 Antibody IF HeLa cells 18933-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 18933-1-AP(BMP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
HT-1080 cells, HeLa cells
Positive IHC detected in:
human lung tissue
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
BMP2 fusion protein Ag13501
Full name:
bone morphogenetic protein 2
Calculated molecular weight:
396aa,44 kDa
Observed molecular weight:
45 kDa, 30 kDa, 18 kDa
GenBank accession number:
BC069214
Gene ID (NCBI):
650
Gene symbol
BMP2
Synonyms
BMP 2, BMP 2A, BMP2, BMP2A, bone morphogenetic protein 2, Bone morphogenetic protein 2A

Background

Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer; 2 monomers then associate to form the active 30 kDa homodimer, which binds to its receptor. There is also a 40-45 kDa form of BMP2, as an amino-terminal propeptide. BMP2 can induce bone formation and regeneration during early embryonic development. It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction.


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