BRAF Polyclonal antibody

BRAF Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20899-1-AP

Synonyms

B RAF1, BRAF, BRAF1, p94, Proto oncogene B Raf, RAFB1



Tested Applications

Positive WB detected inHT-29 cells, Hela cells, Jurkat cells, K-562 cells, NIH/3T3 cells
Positive IHC detected inhuman malignant melanoma tissue, human testis tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 10 publications below

Product Information

The immunogen of 20899-1-AP is BRAF Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRAF fusion protein Ag15014
Full Name v-raf murine sarcoma viral oncogene homolog B1
Calculated molecular weight 766 aa, 84 kDa
Observed molecular weight65 kDa
GenBank accession numberBC101757
Gene symbol BRAF
Gene ID (NCBI) 673
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B-Raf proto-oncogene serine/threonine kinase(BRAF), is a protein belonging to the raf/mil family of serine/threonine protein kinases. BRAF plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. BRAF is associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. BRAF is also associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. BRAF plays a role in the PD-1/PDL-1 pathway. In some cancers, BRAF is activated by rearrangements that fuse its kinase domain to 5' partner genes and then has different molecular weights (PMID: 23890088). BRAF has several isoforms, the calculated molecular weight of BRAF is 84 kDa, but the observed molecular weight is about 65 kDa(isoform).

Protocols

Product Specific Protocols
WB protocol for BRAF antibody 20899-1-APDownload protocol
IHC protocol for BRAF antibody 20899-1-APDownload protocol
IF protocol for BRAF antibody 20899-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Acta Pharmacol Sin

1-Indanone retards cyst development in ADPKD mouse model by stabilizing tubulin and down-regulating anterograde transport of cilia

Authors - Xiao-Wei Li
humanWB

J Eur Acad Dermatol Venereol

Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi.

Authors - T Yuan
humanWB

Front Immunol

Immune Dysfunction Mediated by the ceRNA Regulatory Network in Human Placenta Tissue of Intrahepatic Cholestasis Pregnancy.

Authors - Yuya Wang
humanWB

Life Sci

Network pharmacology and molecular docking reveal the mechanism of Scopoletin against non-small cell lung cancer.

Authors - Chong Yuan
chickenWB

Gene

WT1 suppresses follicle-stimulating hormone-induced progesterone secretion by regulating ERK1/2 pathway in chicken preovulatory granulosa cells.

Authors - Shuixin Tang
humanWB

Biochem Biophys Res Commun

Sex hormone-binding globulin regulates the activity of the ERK pathway in the placentas of patients with gestational diabetes mellitus.

Authors - Xiaoyan Wang