BRD7 Polyclonal antibody, PBS Only

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  WB analysis of HeLa using 51009-2-AP (same clone as 51009-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 51009-2-AP (same clone as 51009-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 51009-2-AP (same clone as 51009-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 51009-2-AP (same clone as 51009-2-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 51009-2-AP (same clone as 51009-2-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 51009-2-AP (same clone as 51009-2-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse cerebellum using 51009-2-AP (same clone as 51009-2-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 51009-2-AP (same clone as 51009-2-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 51009-2-AP (same clone as 51009-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51009-2-AP (BRD7 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 51009-2-AP (same clone as 51009-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51009-2-AP (BRD7 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 51009-2-AP (same clone as 51009-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51009-2-AP (BRD7 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 51009-2-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

BRD7 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 51009-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

51009-2-PBS targets BRD7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3911

  Product name: Recombinant human BRD7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-353 aa of BC050728

  Sequence: MGKKHKKHKSDKHLYEEYVEKPLKLVLKVGGNEVTELSTGSSGHDSSLFEDKNDHDKHKDRKRKKRKKGEKQIPGEEKGRKRRRVKEDKKKRDRDRVENEAEKDLQCHAPVRLDLPPEKPLTSSLAKQEEVEQTPLQEALNQLMRQLQRKDPSAFFSFPVTDFIAPGYSMIIKHPMDFSTMKEKIKNNDYQSIEELKDNFKLMCTNAMIYNKPETIYYKAAKKLLHSGMKILSQERIQSLKQSIDFMADLQKTRKQKDGTDTSQSGEDGGCWQREREDSGDAEAHAFKSPSKENKKKDKDMLEDKFKSNNLEREQEQLDRIVKESGGKLTRRLVNSQCEFERRKPDGTTTLGL

• Full Name: bromodomain containing 7
• Calculated Molecular Weight: 651 aa, 74 kDa
• Observed Molecular Weight: 70-74 kDa
• GenBank Accession Number: BC050728
• Gene Symbol: BRD7
• Gene ID (NCBI): 29117
• RRID: AB_2259226
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NPI1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BRD7 (bromodomain containing 7), is a subunit of poly-bromo-associated BRG1-associated factor (PBAF)-specific Swi/Snf chromatin remodeling complexes.BRD7 is proposed as a tumor suppressor protein, since its identification as an important component of both functional p53 and BRCA1 (breast cancer 1, early onset) pathways. BRD7 could down-regulate the GSK3beta phosphotransferase activity as dephosphorylation of GSK3B at 'Tyr-216', thus activate the Wnt signaling pathway in a DVL1-dependent manner.